CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016954
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Palmitoyltransferase ZDHHC13 
Protein Synonyms/Alias
 Huntingtin-interacting protein 14-related protein; HIP14-related protein; Huntingtin-interacting protein HIP3RP; Putative MAPK-activating protein PM03; Putative NF-kappa-B-activating protein 209; Zinc finger DHHC domain-containing protein 13; DHHC-13 
Gene Name
 ZDHHC13 
Gene Synonyms/Alias
 HIP14L; HIP3RP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33GICAHENKELANAREubiquitination[1, 2, 3]
55SSNCDIVKATQYGIFubiquitination[1]
80YDVRQPDKENVSLLHubiquitination[4, 5]
214PSLNVVDKIHQNTPLubiquitination[6]
249SLDIQNVKGETPLDMubiquitination[6, 7]
274HMLKTEAKMRANQKFubiquitination[2]
566QKQSKHMKQTLSLRKubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Palmitoyltransferase for HD and GAD2 (By similarity). Mediates Mg(2+) transport (By similarity). 
Sequence Annotation
 REPEAT 81 110 ANK 1.
 REPEAT 115 144 ANK 2.
 REPEAT 148 177 ANK 3.
 REPEAT 181 211 ANK 4.
 REPEAT 216 245 ANK 5.
 REPEAT 249 277 ANK 6.
 ZN_FING 426 476 DHHC-type.
 ACT_SITE 456 456 S-palmitoyl cysteine intermediate (By  
Keyword
 Acyltransferase; Alternative splicing; ANK repeat; Complete proteome; Cytoplasmic vesicle; Golgi apparatus; Ion transport; Lipoprotein; Magnesium; Membrane; Metal-binding; Palmitate; Polymorphism; Reference proteome; Repeat; Transferase; Transmembrane; Transmembrane helix; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 622 AA 
Protein Sequence
MEGPGLGSQC RNHSHGPHPP GFGRYGICAH ENKELANARE ALPLIEDSSN CDIVKATQYG 60
IFERCKELVE AGYDVRQPDK ENVSLLHWAA INNRLDLVKF YISKGAVVDQ LGGDLNSTPL 120
HWAIRQGHLP MVILLLQHGA DPTLIDGEGF SSIHLAVLFQ HMPIIAYLIS KGQSVNMTDV 180
NGQTPLMLSA HKVIGPEPTG FLLKFNPSLN VVDKIHQNTP LHWAVAAGNV NAVDKLLEAG 240
SSLDIQNVKG ETPLDMALQN KNQLIIHMLK TEAKMRANQK FRLWRWLQKC ELFLLLMLSV 300
ITMWAIGYIL DFNSDSWLLK GCLLVTLFFL TSLFPRFLVG YKNLVYLPTA FLLSSVFWIF 360
MTWFILFFPD LAGAPFYFSF IFSIVAFLYF FYKTWATDPG FTKASEEEKK VNIITLAETG 420
SLDFRTFCTS CLIRKPLRSL HCHVCNCCVA RYDQHCLWTG RCIGFGNHHY YIFFLFFLSM 480
VCGWIIYGSF IYLSSHCATT FKEDGLWTYL NQIVACSPWV LYILMLATFH FSWSTFLLLN 540
QLFQIAFLGL TSHERISLQK QSKHMKQTLS LRKTPYNLGF MQNLADFFQC GCFGLVKPCV 600
VDWTSQYTMV FHPAREKVLR SV 622 
Gene Ontology
 GO:0030660; C:Golgi-associated vesicle membrane; ISS:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0015095; F:magnesium ion transmembrane transporter activity; ISS:UniProtKB.
 GO:0016409; F:palmitoyltransferase activity; ISS:UniProtKB.
 GO:0004871; F:signal transducer activity; IMP:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR001594; Znf_DHHC_palmitoyltrfase. 
Pfam
 PF12796; Ank_2
 PF01529; zf-DHHC 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50216; ZF_DHHC 
PRINTS