CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000900
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 C-Jun-amino-terminal kinase-interacting protein 4 
Protein Synonyms/Alias
 JIP-4; JNK-interacting protein 4; Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1 
Gene Name
 SPAG9 
Gene Synonyms/Alias
 HSS; KIAA0516; MAPK8IP4; SYD1; HLC6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
233TPGSEQWKFQELSQPubiquitination[1]
653FGWSLPQKYKQVTNGubiquitination[1]
1227HGHRDAVKFFVAVPGubiquitination[1, 2]
1305PLEPSVTKAERSHLIubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg- interaction. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 109 109 Phosphoserine.
 MOD_RES 183 183 Phosphoserine.
 MOD_RES 185 185 Phosphoserine.
 MOD_RES 194 194 Phosphoserine.
 MOD_RES 203 203 Phosphoserine.
 MOD_RES 217 217 Phosphothreonine.
 MOD_RES 244 244 Phosphothreonine (By similarity).
 MOD_RES 251 251 Phosphoserine.
 MOD_RES 265 265 Phosphoserine.
 MOD_RES 268 268 Phosphoserine.
 MOD_RES 272 272 Phosphoserine.
 MOD_RES 292 292 Phosphothreonine (By similarity).
 MOD_RES 311 311 Phosphoserine.
 MOD_RES 329 329 Phosphoserine.
 MOD_RES 332 332 Phosphoserine.
 MOD_RES 347 347 Phosphoserine.
 MOD_RES 348 348 Phosphothreonine.
 MOD_RES 365 365 Phosphothreonine (By similarity).
 MOD_RES 418 418 Phosphothreonine.
 MOD_RES 586 586 Phosphothreonine.
 MOD_RES 730 730 Phosphoserine.
 MOD_RES 733 733 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1321 AA 
Protein Sequence
MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN 60
LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR 120
VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH 180
QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP 240
RSHTSLKVSN SPEPQKAVEQ EDELSDVSQG GSKATTPAST ANSDVATIPT DTPLKEENEG 300
FVKVTDAPNK SEISKHIEVQ VAQETRNVST GSAENEEKSE VQAIIESTPE LDMDKDLSGY 360
KGSSTPTKGI ENKAFDRNTE SLFEELSSAG SGLIGDVDEG ADLLGMGREV ENLILENTQL 420
LETKNALNIV KNDLIAKVDE LTCEKDVLQG ELEAVKQAKL KLEEKNRELE EELRKARAEA 480
EDARQKAKDD DDSDIPTAQR KRFTRVEMAR VLMERNQYKE RLMELQEAVR WTEMIRASRE 540
NPAMQEKKRS SIWQFFSRLF SSSSNTTKKP EPPVNLKYNA PTSHVTPSVK KRSSTLSQLP 600
GDKSKAFDFL SEETEASLAS RREQKREQYR QVKAHVQKED GRVQAFGWSL PQKYKQVTNG 660
QGENKMKNLP VPVYLRPLDE KDTSMKLWCA VGVNLSGGKT RDGGSVVGAS VFYKDVAGLD 720
TEGSKQRSAS QSSLDKLDQE LKEQQKELKN QEELSSLVWI CTSTHSATKV LIIDAVQPGN 780
ILDSFTVCNS HVLCIASVPG ARETDYPAGE DLSESGQVDK ASLCGSMTSN SSAETDSLLG 840
GITVVGCSAE GVTGAATSPS TNGASPVMDK PPEMEAENSE VDENVPTAEE ATEATEGNAG 900
SAEDTVDISQ TGVYTEHVFT DPLGVQIPED LSPVYQSSND SDAYKDQISV LPNEQDLVRE 960
EAQKMSSLLP TMWLGAQNGC LYVHSSVAQW RKCLHSIKLK DSILSIVHVK GIVLVALADG 1020
TLAIFHRGVD GQWDLSNYHL LDLGRPHHSI RCMTVVHDKV WCGYRNKIYV VQPKAMKIEK 1080
SFDAHPRKES QVRQLAWVGD GVWVSIRLDS TLRLYHAHTY QHLQDVDIEP YVSKMLGTGK 1140
LGFSFVRITA LMVSCNRLWV GTGNGVIISI PLTETNKTSG VPGNRPGSVI RVYGDENSDK 1200
VTPGTFIPYC SMAHAQLCFH GHRDAVKFFV AVPGQVISPQ SSSSGTDLTG DKAGPSAQEP 1260
GSQTPLKSML VISGGEGYID FRMGDEGGES ELLGEDLPLE PSVTKAERSH LIVWQVMYGN 1320
E 1321 
Gene Ontology
 GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0007257; P:activation of JUN kinase activity; IEA:Compara.
 GO:0042692; P:muscle cell differentiation; TAS:Reactome.
 GO:0090074; P:negative regulation of protein homodimerization activity; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IMP:UniProtKB.
 GO:0051149; P:positive regulation of muscle cell differentiation; TAS:Reactome.
 GO:0045666; P:positive regulation of neuron differentiation; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0042147; P:retrograde transport, endosome to Golgi; IDA:MGI.
 GO:0007283; P:spermatogenesis; TAS:ProtInc.
 GO:0051146; P:striated muscle cell differentiation; IEA:Compara. 
Interpro
 IPR019143; JNK/Rab-associated_protein-1_N.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF09744; Jnk-SapK_ap_N 
SMART
  
PROSITE
  
PRINTS