CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032042
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutathione synthetase 
Protein Synonyms/Alias
 cDNA FLJ50601, highly similar to Glutathione synthetase (EC 6.3.2.3) 
Gene Name
 GSS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13ADGSPALKQIEINTIubiquitination[1, 2, 3, 4, 5]
44HVLSVLSKTKEAGKIubiquitination[3]
58ILSNNPSKGLALGIAubiquitination[1, 3, 4]
85VLLIAQEKERNIFDQubiquitination[1]
117TFEDISEKGSLDQDRubiquitination[1, 3, 4, 5]
165LERSHAAKCPDIATQubiquitination[2, 3, 5]
177ATQLAGTKKVQQELSubiquitination[6]
178TQLAGTKKVQQELSRubiquitination[3, 5]
236APSRFVLKPQREGGGubiquitination[3]
256EEMVQALKQLKDSEEubiquitination[1, 2, 3, 4, 5]
272ASYILMEKIEPEPFEubiquitination[3]
324VGHLLRTKAIEHADGubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 346 AA 
Protein Sequence
MFQRSADGSP ALKQIEINTI SASFGGLASR TPAVHRHVLS VLSKTKEAGK ILSNNPSKGL 60
ALGIAKAWEL YGSPNALVLL IAQEKERNIF DQRAIENELL ARNIHVIRRT FEDISEKGSL 120
DQDRRLFVDG QEIAVVYFRD GYMPRQYSLQ NWEARLLLER SHAAKCPDIA TQLAGTKKVQ 180
QELSRPGMLE MLLPGQPEAV ARLRATFAGL YSLDVGEEGD QAIAEALAAP SRFVLKPQRE 240
GGGNNLYGEE MVQALKQLKD SEERASYILM EKIEPEPFEN CLLRPGSPAR VVQCISELGI 300
FGVYVRQEKT LVMNKHVGHL LRTKAIEHAD GGVAAGVAVL DNPYPV 346 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004363; F:glutathione synthase activity; IEA:Compara.
 GO:0016594; F:glycine binding; IEA:Compara.
 GO:0042277; F:peptide binding; IEA:Compara.
 GO:0007568; P:aging; IEA:Compara.
 GO:0043200; P:response to amino acid stimulus; IEA:Compara.
 GO:0046686; P:response to cadmium ion; IEA:Compara.
 GO:0031667; P:response to nutrient levels; IEA:Compara.
 GO:0034612; P:response to tumor necrosis factor; IEA:Compara.
 GO:0009410; P:response to xenobiotic stimulus; IEA:Compara. 
Interpro
 IPR004887; Glutathione_synth_subst-bd_euk.
 IPR014042; Glutathione_synthase_a-hlx_euk.
 IPR014709; Glutathione_synthase_dom.
 IPR005615; Glutathione_synthase_euk.
 IPR014049; Glutathione_synthase_N_euk.
 IPR016185; PreATP-grasp_dom. 
Pfam
 PF03917; GSH_synth_ATP
 PF03199; GSH_synthase 
SMART
  
PROSITE
  
PRINTS