CPLM-003772

Genbank Nucleotide ID

60 kDa SS-A/Ro ribonucleoprotein

Protein Synonyms/Alias

60 kDa Ro protein; 60 kDa ribonucleoprotein Ro; RoRNP; Ro 60 kDa autoantigen; Sjoegren syndrome antigen A2; Sjoegren syndrome type A antigen; SS-A; TROVE domain family member 2

Homo sapiens (Human)

Position	Peptide	Type	References
49	EGGTYYIKEQKLGLE	ubiquitination	[1, 2]
52	TYYIKEQKLGLENAE	ubiquitination	[2]
140	KDLKESMKCGMWGRA	ubiquitination	[1, 2]
180	QRNGWSHKDLLRLSH	ubiquitination	[2]
189	LLRLSHLKPSSEGLA	ubiquitination	[1]
207	KYITKGWKEVHELYK	ubiquitination	[2]
224	ALSVETEKLLKYLEA	acetylation	[3]
224	ALSVETEKLLKYLEA	ubiquitination	[4, 5, 6]
227	VETEKLLKYLEAVEK	ubiquitination	[4, 6]
234	KYLEAVEKVKRTRDE	ubiquitination	[2]
236	LEAVEKVKRTRDELE	ubiquitination	[2]
264	HLLTNHLKSKEVWKA	ubiquitination	[2]
266	LTNHLKSKEVWKALL	ubiquitination	[2]
287	ALLRNLGKMTANSVL	ubiquitination	[2]
307	EVSLVCEKLCNEKLL	ubiquitination	[2]
312	CEKLCNEKLLKKARI	ubiquitination	[2]
342	HGLRGKLKWRPDEEI	ubiquitination	[1, 2, 4, 6]
359	ALDAAFYKTFKTVEP	acetylation	[3]
359	ALDAAFYKTFKTVEP	ubiquitination	[1, 4, 6]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. May stabilize some of these RNAs and protect them from degradation.

DOMAIN 16 369 TROVE.
REGION 120 284 RNA-binding (By similarity).
REGION 361 538 VWFA-like domain (By similarity).
METAL 378 378 Divalent metal cation (By similarity).
METAL 380 380 Divalent metal cation (By similarity).
METAL 445 445 Divalent metal cation (By similarity).
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 19 19 Phosphoserine.
MOD_RES 224 224 N6-acetyllysine.
MOD_RES 359 359 N6-acetyllysine.

Acetylation; Alternative splicing; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:HPA.
GO:0005634; C:nucleus; IDA:HPA.
GO:0030529; C:ribonucleoprotein complex; IDA:UniProtKB.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0003723; F:RNA binding; TAS:ProtInc.
GO:0060271; P:cilium morphogenesis; IEA:Compara.
GO:0006383; P:transcription from RNA polymerase III promoter; TAS:ProtInc.

IPR008858; TROVE.
IPR018698; VWA-like_dom.

PF09967; DUF2201
PF05731; TROVE