UniProt Accession

 IWS1_YEAST; Q06505; D6W4C9 

Genbank Protein ID

 U40829; AY558069; BK006949 

Genbank Nucleotide ID

 AAB68274.1; AAS56395.1; DAA11545.1 

Protein Name

 Transcription factor SPN1 

Protein Synonyms/Alias

 Interacts with SPT6 protein 1; Suppresses postrecruitment functions protein 1 

Gene Name

 SPN1 

Gene Synonyms/Alias

 IWS1; YPR133C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
25	DGTASSQKSTINAEN	acetylation	[1]
25	DGTASSQKSTINAEN	ubiquitination	[2]
36	NAENENTKQNQSMEP	ubiquitination	[2]
369	AQTTTDYKYAPVSNL	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post- recruitment and recruitment steps of transcription. 

Sequence Annotation

 DOMAIN 219 296 TFIIS N-terminal.
 MOD_RES 15 15 Phosphothreonine.
 MOD_RES 23 23 Phosphoserine; by ATM or ATR.
 MOD_RES 40 40 Phosphoserine.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 86 86 Phosphothreonine.
 MOD_RES 89 89 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 410 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IC:SGD.
 GO:0000991; F:core RNA polymerase II binding transcription factor activity; IPI:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0001076; F:RNA polymerase II transcription factor binding transcription factor activity; IPI:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD.
 GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IGI:SGD. 

Interpro

 IPR017923; TFIIS_N. 

Pfam

 PF08711; Med26 

SMART

  

PROSITE

 PS51319; TFIIS_N 

PRINTS