UniProt Accession

 TPM4_HUMAN; P67936; P07226; Q15659; Q5U0D9; Q9BU85; Q9H8Q3; Q9UCS1; Q9UCS2; Q9UCS3; Q9UCS4 

Genbank Protein ID

 X05276; AK023385; AK315076; BT019634; CH471106; BC002827; BC037576; BC067225; M12127 

Genbank Nucleotide ID

 CAA28888.1; BAB14554.1; BAG37545.1; AAV38440.1; EAW84521.1; AAH02827.1; AAH37576.1; AAH67225.1; AAA36774.1 

Protein Name

 Tropomyosin alpha-4 chain 

Protein Synonyms/Alias

 TM30p1; Tropomyosin-4 

Gene Name

 TPM4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	LNSLEAVKRKIQALQ	acetylation	[1]
11	LNSLEAVKRKIQALQ	ubiquitination	[1, 2, 3, 4]
13	SLEAVKRKIQALQQQ	ubiquitination	[1, 2, 4, 5, 6]
113	EIQEMQLKEAKHIAE	ubiquitination	[6]
116	EMQLKEAKHIAEEAD	ubiquitination	[6]
132	KYEEVARKLVILEGE	ubiquitination	[7]
169	KNVTNNLKSLEAASE	ubiquitination	[3, 6, 7, 8]
177	SLEAASEKYSEKEDK	acetylation	[9]
177	SLEAASEKYSEKEDK	ubiquitination	[5, 6]
184	KYSEKEDKYEEEIKL	ubiquitination	[5, 6]
190	DKYEEEIKLLSDKLK	ubiquitination	[7, 8]
212	FAERTVAKLEKTIDD	ubiquitination	[4]
215	RTVAKLEKTIDDLEE	acetylation	[9]
215	RTVAKLEKTIDDLEE	ubiquitination	[4, 5]
228	EEKLAQAKEENVGLH	ubiquitination	[6]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [9] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 177 177 N6-acetyllysine.
 MOD_RES 215 215 N6-acetyllysine.  

Keyword

 Acetylation; Actin-binding; Alternative splicing; Calcium; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Metal-binding; Muscle protein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 248 AA 

Protein Sequence

Gene Ontology

 GO:0030863; C:cortical cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031941; C:filamentous actin; IEA:Compara.
 GO:0005862; C:muscle thin filament tropomyosin; TAS:ProtInc.
 GO:0002102; C:podosome; IEA:Compara.
 GO:0001725; C:stress fiber; IDA:MGI.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0008307; F:structural constituent of muscle; TAS:ProtInc.
 GO:0030049; P:muscle filament sliding; TAS:Reactome.
 GO:0006979; P:response to oxidative stress; IEP:BHF-UCL. 

Interpro

 IPR000533; Tropomyosin. 

Pfam

 PF00261; Tropomyosin 

SMART

  

PROSITE

 PS00326; TROPOMYOSIN 

PRINTS

 PR00194; TROPOMYOSIN.