CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008596
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Negative regulator of sporulation MDS3 
Protein Synonyms/Alias
 MCK1 dosage suppressor 3 
Gene Name
 MDS3 
Gene Synonyms/Alias
 YGL197W; G1307 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
128QRTEELLKAESSSAKubiquitination[1]
135KAESSSAKADNDTNEubiquitination[1]
319QIDEYLGKDSSKIKEubiquitination[1]
942LDGQMLGKSLKEGSTubiquitination[1]
945QMLGKSLKEGSTSQYubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1. 
Sequence Annotation
 REPEAT 171 226 Kelch 1.
 REPEAT 234 287 Kelch 2.
 REPEAT 371 419 Kelch 3.
 MOD_RES 472 472 Phosphoserine.
 MOD_RES 475 475 Phosphoserine.
 MOD_RES 621 621 Phosphoserine.
 MOD_RES 693 693 Phosphoserine.
 MOD_RES 698 698 Phosphoserine.
 MOD_RES 744 744 Phosphoserine.
 MOD_RES 747 747 Phosphoserine.
 MOD_RES 756 756 Phosphoserine.
 MOD_RES 757 757 Phosphoserine.
 MOD_RES 781 781 Phosphoserine.
 MOD_RES 785 785 Phosphothreonine.
 MOD_RES 787 787 Phosphoserine.
 MOD_RES 900 900 Phosphoserine.
 MOD_RES 904 904 Phosphoserine.
 MOD_RES 930 930 Phosphothreonine.
 MOD_RES 1187 1187 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Kelch repeat; Meiosis; Phosphoprotein; Reference proteome; Repeat; Sporulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1487 AA 
Protein Sequence
MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR SNIFVHGGLT 60
IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK ETFFLDLMSR TWYRVKTSFD 120
QRTEELLKAE SSSAKADNDT NEIRTDIKKG KSLESPLKER LFHSLCYLDG CLYIFGGLTV 180
SPQSGYELIA TNELWKLDLN TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG 240
LNNMDQPVKK IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN 300
VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL PKKLLNVPYE 360
LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS RVATACPDCD INKHRFWRVF 420
VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS TFGLPLVNIF NKTIQLPHHK ISASSLPIPI 480
ENFAKHKDTP LKKVSFTSSA TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG 540
KPLSDFEFIT SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP 600
HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN ADAKAPNTPS 660
TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS NSIYRPIRKT ESSSTTSSSN 720
GMIFRVPFKE KAAVTSNTEA LLESNLSLQE LSRRRSSLMS IPSGELLRSS ISEAEHQRRA 780
SHPLTSSPLF EDSGTPCGKQ LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG 840
NSISSRSTSD SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS 900
NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS QYTQPRMNSF 960
PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL EPLLTPRSLY MPWPTSTVRA 1020
FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY EIPLLYKLIL EVLYSILAKK EESLSLICTS 1080
LMETFRTKTL NSYKGDEEKT NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ 1140
SSSTQESSGS ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR 1200
RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF NLHLFDMNYG 1260
SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD ARFRNKEDDK VTKDISNDKK 1320
RNYLPHEKNN LKAKEGKETR DVREEEEEFD FGLGMLSLNK IKREAKHVDK VDDSVDPLFK 1380
SSAFPQSPIR AYGSTTRTSS ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY 1440
RTTVLVNDIR LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP 1487 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0042174; P:negative regulation of sporulation resulting in formation of a cellular spore; IGI:SGD.
 GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
 GO:0031929; P:TOR signaling cascade; IMP:SGD. 
Interpro
 IPR015915; Kelch-typ_b-propeller. 
Pfam
  
SMART
  
PROSITE
  
PRINTS