CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010998
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 60S ribosomal protein L6 
Protein Synonyms/Alias
 Neoplasm-related protein C140; Tax-responsive enhancer element-binding protein 107; TaxREB107 
Gene Name
 RPL6 
Gene Synonyms/Alias
 TXREB1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MAGEKVEKPDTKacetylation[1]
5***MAGEKVEKPDTKubiquitination[2]
8MAGEKVEKPDTKEKKubiquitination[2]
87VEKKKKEKVLATVTKubiquitination[2]
94KVLATVTKPVGGDKNubiquitination[2, 3, 4]
100TKPVGGDKNGGTRVVubiquitination[2, 3, 4]
130RKLLSHGKKPFSQHVubiquitination[2, 3, 5, 6]
131KLLSHGKKPFSQHVRubiquitination[2, 3, 4]
192PLRRTHQKFVIATSTubiquitination[2]
207KIDISNVKIPKHLTDubiquitination[2, 3, 4, 5, 6, 7]
210ISNVKIPKHLTDAYFacetylation[1]
210ISNVKIPKHLTDAYFubiquitination[2, 3]
218HLTDAYFKKKKLRKPacetylation[1]
218HLTDAYFKKKKLRKPubiquitination[2, 4]
219LTDAYFKKKKLRKPRubiquitination[3]
220TDAYFKKKKLRKPRHubiquitination[2]
237GEIFDTEKEKYEITEubiquitination[2, 4]
239IFDTEKEKYEITEQRacetylation[1]
239IFDTEKEKYEITEQRubiquitination[2, 3]
247YEITEQRKIDQKAVDubiquitination[2]
251EQRKIDQKAVDSQILubiquitination[2, 4]
260VDSQILPKIKAIPQLubiquitination[2]
262SQILPKIKAIPQLQGubiquitination[2, 3, 4, 5, 6, 7]
285TNGIYPHKLVF****ubiquitination[2, 3, 4, 8, 9, 10]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Specifically binds to domain C of the Tax-responsive enhancer element in the long terminal repeat of HTLV-I. 
Sequence Annotation
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 218 218 N6-acetyllysine.
 MOD_RES 239 239 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosomal protein. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 288 AA 
Protein Sequence
MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS 60
AMYSRKAMYK RKYSAAKSKV EKKKKEKVLA TVTKPVGGDK NGGTRVVKLR KMPRYYPTED 120
VPRKLLSHGK KPFSQHVRKL RASITPGTIL IILTGRHRGK RVVFLKQLAS GLLLVTGPLV 180
LNRVPLRRTH QKFVIATSTK IDISNVKIPK HLTDAYFKKK KLRKPRHQEG EIFDTEKEKY 240
EITEQRKIDQ KAVDSQILPK IKAIPQLQGY LRSVFALTNG IYPHKLVF 288 
Gene Ontology
 GO:0022625; C:cytosolic large ribosomal subunit; IDA:UniProtKB.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0003735; F:structural constituent of ribosome; NAS:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0006355; P:regulation of transcription, DNA-dependent; TAS:ProtInc.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
 GO:0006414; P:translational elongation; TAS:Reactome.
 GO:0006413; P:translational initiation; TAS:Reactome.
 GO:0006415; P:translational termination; TAS:Reactome.
 GO:0019083; P:viral transcription; TAS:Reactome. 
Interpro
 IPR014722; Rib_L2_dom2.
 IPR005568; Ribosomal_L6_N.
 IPR000915; Ribosomal_L6E. 
Pfam
 PF01159; Ribosomal_L6e
 PF03868; Ribosomal_L6e_N 
SMART
  
PROSITE
 PS01170; RIBOSOMAL_L6E 
PRINTS