CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031920
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin-linked protein kinase 
Protein Synonyms/Alias
 cDNA FLJ53825, highly similar to Integrin-linked protein kinase 1 (EC 2.7.11.1) 
Gene Name
 ILK 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20LLRERAEKMGQNLNRubiquitination[1]
31NLNRIPYKDTFWKGTubiquitination[1, 2]
36PYKDTFWKGTTRTRPubiquitination[3]
50PRNGTLNKHSGIDFKubiquitination[1]
57KHSGIDFKQLNFLTKubiquitination[1, 2, 3, 4, 5]
64KQLNFLTKLNENHSGubiquitination[1, 3]
75NHSGELWKGRWQGNDubiquitination[1, 3, 4, 5]
86QGNDIVVKVLKVRDWubiquitination[1, 2, 3, 4, 6]
207RISMADVKFSFQCPGubiquitination[1, 4]
292PHVCKLMKICMNEDPacetylation[6, 7, 8]
292PHVCKLMKICMNEDPubiquitination[1]
304EDPAKRPKFDMIVPIubiquitination[1, 2, 3, 5]
314MIVPILEKMQDK***ubiquitination[1, 3, 4, 5]
318ILEKMQDK*******ubiquitination[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Integrin; Kinase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 318 AA 
Protein Sequence
MPVDKAKAPL RELLRERAEK MGQNLNRIPY KDTFWKGTTR TRPRNGTLNK HSGIDFKQLN 60
FLTKLNENHS GELWKGRWQG NDIVVKVLKV RDWSTRKSRD FNEECPRLRI FSHPNVLPVL 120
GACQSPPAPH PTLITHWMPY GSLYNVLHEG TNFVVDQSQA VKFALDMARG MAFLHTLEPL 180
IPRHALNSRS VMIDEDMTAR ISMADVKFSF QCPGRMYAPA WVAPEALQKK PEDTNRRSAD 240
MWSFAVLLWE LVTREVPFAD LSNMEIGMKV ALEGLRPTIP PGISPHVCKL MKICMNEDPA 300
KRPKFDMIVP ILEKMQDK 318 
Gene Ontology
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0043034; C:costamere; IEA:Compara.
 GO:0043198; C:dendritic shaft; IEA:Compara.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0043195; C:terminal bouton; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:Compara.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0001658; P:branching involved in ureteric bud morphogenesis; IEA:Compara.
 GO:0007569; P:cell aging; IEA:Compara.
 GO:0007050; P:cell cycle arrest; IEA:Compara.
 GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; IEA:Compara.
 GO:0043206; P:extracellular fibril organization; IEA:Compara.
 GO:0007229; P:integrin-mediated signaling pathway; IEA:UniProtKB-KW.
 GO:0032288; P:myelin assembly; IEA:Compara.
 GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0006469; P:negative regulation of protein kinase activity; IEA:Compara.
 GO:0014912; P:negative regulation of smooth muscle cell migration; IEA:Compara.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0048812; P:neuron projection morphogenesis; IEA:Compara.
 GO:0018105; P:peptidyl-serine phosphorylation; IEA:Compara.
 GO:0045773; P:positive regulation of axon extension; IEA:Compara.
 GO:0030513; P:positive regulation of BMP signaling pathway; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0001954; P:positive regulation of cell-matrix adhesion; IEA:Compara.
 GO:0050775; P:positive regulation of dendrite morphogenesis; IEA:Compara.
 GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
 GO:0045663; P:positive regulation of myoblast differentiation; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0032956; P:regulation of actin cytoskeleton organization; IEA:Compara.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IEA:Compara. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom. 
Pfam
 PF07714; Pkinase_Tyr 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM 
PRINTS