CPLM-012178

Genbank Nucleotide ID

GDP-L-fucose synthase

Protein Synonyms/Alias

GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; Protein FX; Red cell NADP(H)-binding protein; Short-chain dehydrogenase/reductase family 4E member 1

Homo sapiens (Human)

Position	Peptide	Type	References
21	GGSGLVGKAIQKVVA	ubiquitination	[1, 2, 3, 4, 5, 6]
44	DWVFVSSKDADLTDT	ubiquitination	[2, 5]
89	YNLDFWRKNVHMNDN	ubiquitination	[3, 4]
147	NFGYSYAKRMIDVQN	acetylation	[7]

[1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

Catalyzes the two-step NADP-dependent conversion of GDP- 4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.

NP_BIND 14 20 NADP.
NP_BIND 170 173 NADP.
ACT_SITE 143 143 Proton donor/acceptor (By similarity).
BINDING 147 147 NADP.
BINDING 186 186 NADP.
BINDING 194 194 Substrate.
BINDING 208 208 Substrate.
BINDING 215 215 Substrate.
BINDING 277 277 Substrate.

3D-structure; Complete proteome; Direct protein sequencing; Isomerase; Multifunctional enzyme; NADP; Oxidoreductase; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IC:UniProtKB.
GO:0050662; F:coenzyme binding; IEA:InterPro.
GO:0009055; F:electron carrier activity; TAS:UniProtKB.
GO:0042356; F:GDP-4-dehydro-D-rhamnose reductase activity; TAS:UniProtKB.
GO:0050577; F:GDP-L-fucose synthase activity; IDA:UniProtKB.
GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
GO:0042351; P:'de novo' GDP-L-fucose biosynthetic process; IDA:UniProtKB.
GO:0019835; P:cytolysis; IEA:Compara.
GO:0019673; P:GDP-mannose metabolic process; IDA:UniProtKB.
GO:0007159; P:leukocyte cell-cell adhesion; NAS:UniProtKB.

IPR001509; Epimerase_deHydtase.
IPR016040; NAD(P)-bd_dom.