CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016946
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TIR domain-containing adapter molecule 1 
Protein Synonyms/Alias
 TICAM-1; Proline-rich, vinculin and TIR domain-containing protein B; Putative NF-kappa-B-activating protein 502H; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta; MyD88-3; TIR domain-containing adapter protein inducing IFN-beta 
Gene Name
 TICAM1 
Gene Synonyms/Alias
 PRVTIRB; TRIF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
65RISLEALKADAVARLubiquitination[1]
707GSQAPEDKTQEAE**ubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF- kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein- interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. 
Sequence Annotation
 DOMAIN 390 460 TIR.
 REGION 512 712 Sufficient to induce apoptosis.
 MOTIF 84 91 TRAF6-binding.
 MOTIF 248 255 TRAF6-binding.
 MOTIF 299 309 TRAF6-binding.  
Keyword
 3D-structure; Antiviral defense; Apoptosis; Complete proteome; Disease mutation; Immunity; Inflammatory response; Innate immunity; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 712 AA 
Protein Sequence
MACTGPSLPS AFDILGAAGQ DKLLYLKHKL KTPRPGCQGQ DLLHAMVLLK LGQETEARIS 60
LEALKADAVA RLVARQWAGV DSTEDPEEPP DVSWAVARLY HLLAEEKLCP ASLRDVAYQE 120
AVRTLSSRDD HRLGELQDEA RNRCGWDIAG DPGSIRTLQS NLGCLPPSSA LPSGTRSLPR 180
PIDGVSDWSQ GCSLRSTGSP ASLASNLEIS QSPTMPFLSL HRSPHGPSKL CDDPQASLVP 240
EPVPGGCQEP EEMSWPPSGE IASPPELPSS PPPGLPEVAP DATSTGLPDT PAAPETSTNY 300
PVECTEGSAG PQSLPLPILE PVKNPCSVKD QTPLQLSVED TTSPNTKPCP PTPTTPETSP 360
PPPPPPPSST PCSAHLTPSS LFPSSLESSS EQKFYNFVIL HARADEHIAL RVREKLEALG 420
VPDGATFCED FQVPGRGELS CLQDAIDHSA FIILLLTSNF DCRLSLHQVN QAMMSNLTRQ 480
GSPDCVIPFL PLESSPAQLS SDTASLLSGL VRLDEHSQIF ARKVANTFKP HRLQARKAMW 540
RKEQDTRALR EQSQHLDGER MQAAALNAAY SAYLQSYLSY QAQMEQLQVA FGSHMSFGTG 600
APYGARMPFG GQVPLGAPPP FPTWPGCPQP PPLHAWQAGT PPPPSPQPAA FPQSLPFPQS 660
PAFPTASPAP PQSPGLQPLI IHHAQMVQLG LNNHMWNQRG SQAPEDKTQE AE 712 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0010008; C:endosome membrane; TAS:Reactome.
 GO:0004871; F:signal transducer activity; IMP:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0007249; P:I-kappaB kinase/NF-kappaB cascade; TAS:Reactome.
 GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IEA:Compara.
 GO:0002281; P:macrophage activation involved in immune response; IEA:Compara.
 GO:0030890; P:positive regulation of B cell proliferation; IEA:Compara.
 GO:0045080; P:positive regulation of chemokine biosynthetic process; IEA:Compara.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0045359; P:positive regulation of interferon-beta biosynthetic process; IEA:Compara.
 GO:0032755; P:positive regulation of interleukin-6 production; IEA:Compara.
 GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IEA:Compara.
 GO:0045429; P:positive regulation of nitric oxide biosynthetic process; IEA:Compara.
 GO:0032092; P:positive regulation of protein binding; IEA:Compara.
 GO:0031398; P:positive regulation of protein ubiquitination; IEA:Compara.
 GO:0032760; P:positive regulation of tumor necrosis factor production; IEA:Compara.
 GO:0032481; P:positive regulation of type I interferon production; IEA:Compara.
 GO:0043496; P:regulation of protein homodimerization activity; IEA:Compara.
 GO:0043330; P:response to exogenous dsRNA; IEA:Compara.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR025735; RHIM_dom.
 IPR017278; Tol-interleuk_rcpt_adapt_Ticam. 
Pfam
 PF12721; RHIM 
SMART
  
PROSITE
 PS50104; TIR 
PRINTS