UniProt Accession

 RRP46_YEAST; P53256; D6VUM7; Q6TQU1 

Genbank Protein ID

 Z72880; AY389297; BK006941 

Genbank Nucleotide ID

 CAA97098.1; AAQ97229.1; DAA08188.1 

Protein Name

 Exosome complex component RRP46 

Protein Synonyms/Alias

 Ribosomal RNA-processing protein 46 

Gene Name

 RRP46 

Gene Synonyms/Alias

 YGR095C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
37	VTGPIEPKARQELPT	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP46 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Exosome; Nucleus; Reference proteome; RNA-binding; rRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0000177; C:cytoplasmic exosome (RNase complex); IDA:SGD.
 GO:0000176; C:nuclear exosome (RNase complex); IDA:SGD.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0070651; P:nonfunctional rRNA decay; IC:SGD.
 GO:0071042; P:nuclear polyadenylation-dependent mRNA catabolic process; IMP:SGD.
 GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IMP:SGD.
 GO:0071038; P:nuclear polyadenylation-dependent tRNA catabolic process; IDA:SGD.
 GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; IC:SGD.
 GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IC:SGD.
 GO:0071051; P:polyadenylation-dependent snoRNA 3'-end processing; IC:SGD. 

Interpro

 IPR001247; ExoRNase_PH_dom1.
 IPR015847; ExoRNase_PH_dom2.
 IPR027408; PNPase/RNase_PH_dom.
 IPR020568; Ribosomal_S5_D2-typ_fold. 

Pfam

 PF01138; RNase_PH
 PF03725; RNase_PH_C 

SMART

  

PROSITE

  

PRINTS