CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023415
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mediator of RNA polymerase II transcription subunit 1 
Protein Synonyms/Alias
 Mediator complex subunit 1; dTRAP220 
Gene Name
 MED1 
Gene Synonyms/Alias
 Trap220; CG7162 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
650SDHDIADKYKNIWKDacetylation[1]
889PKHGSSGKPSMSTLKacetylation[1]
1233KKQHTGLKPGVNSGPacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for activated transcription of the MtnA, MtnB and MtnD genes. 
Sequence Annotation
 MOD_RES 830 830 Phosphoserine.
 MOD_RES 834 834 Phosphoserine.
 MOD_RES 854 854 Phosphoserine.
 MOD_RES 858 858 Phosphoserine.  
Keyword
 Activator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1475 AA 
Protein Sequence
MSGSNAKSSG TGFGSHIPSI EEKNKQIQQE TMMEKLRAKY RNKPKSYEEI KKSVRMYFIE 60
KHYPLDPLCK ATLQSALDKL QHYIKVTSRH GLVERLESLS RQLGLKFMED QQLLFISTDM 120
FYVEILLDAA GSLSDVKVHH ECKIEQQSSE LVACLKSGDF ADFTVQLEGL SSIYQLNAEP 180
KVKKKAFVAL QAMETDIQSL YQLHLQGHSG DSYSLMTSSS VGLVLPRRGG HPMRLTYFCP 240
PLHLPEGDPK LASGDFTIDQ VMRSSYGLSA TINLEGSSAN KLQTLPTVTL VRDAQTGLEV 300
PTYAQLNQNN SLLMPATFVL RLNKPMPVCL ESLKALGLPG LDSVATPPGP PTTVLNLIVQ 360
TASKQAIKNT QRGLYVNLPK ETHCYFFTDN RKLQGTLVSS LPFTEPAQVP RIVAFLKKQA 420
LFYTLLASCV REQQKQYNDM DSTVILEVTA VSFNQITVEL QHPYEESLAT VDFLLEDGQP 480
TCSVYCLTNE YELLSQKLTR TARKVVSIPM VIYKLLKCWD EEHEFKLHGA IGPGSGSGAI 540
GGGGMSGGGP VSGVGNNFSQ FSMDTPTPSD GSLPGGGFAN INNLKMDAKS RSLADAFAAS 600
TSAAAAIAGL INLKRETDPQ SGSSASGTTV SGSSSSSGSA KTSDHDIADK YKNIWKDKTP 660
NLKHCVSITP IPGDGKSGSA GGVSGVEVQR TGGIEIIPLN AQAAIAGGGV TASSSATPTT 720
ITITPITGKD PSKDSTKKST AASAGVGVAA KRPHESSTSS SSTSGCSGSG SSMSSSASSG 780
SSDTQKEKKR KKKRDDSPMG PPEKIYSRQN SPAGGADASA TGGVVRKFSS PSSSPKAGGG 840
GQGLMAGVPT ARPSPKHSPV YSSPKHNTAS NSPKSPFGTH SPKHGSSGKP SMSTLKSAAT 900
AATILSPKGD KSSSAVGNTS SGPSASSGSS GATGLVRSFA SVGAPPPPPP IPPLASSSGS 960
ISSSQSLKKE KTSSASGSSS TSSSATAGVA SGGGISPASV AAAVAALKSS QQQMKSVASL 1020
SHLAAGGGLG SYAAPSGAGA SGAAAVVVGA GAGAGAGASG LELSALRKGM AGGAVSLMTS 1080
TAALAPTIPA PTTTVAAGSA ASLVSPVSAV VGQGQETAGA AAAATLATAT ILQQQPQPGA 1140
APTSSCLTTS GGSSDSAGSI NPAGASTEYM VKPSSQEGLK LTINKTGSSK SSGTGSGSSS 1200
SSGLQAKAKS SSSGATSFAG STGSTKKQHT GLKPGVNSGP ASKKATAAVS SATASSSKHF 1260
FQKANSSGNL SSKLSGSGSG GGIPLTKSNS TNSFQEHNAP RRRPSMGALA SGSSGGGSGQ 1320
RKLGSASGGG SGSSGSVSPA LSGSMSQPPP RFDHHTDMMT ILQYASPTMA ASMEGFIKGL 1380
HNKFQIPKLS QRGSGGNTTS GRSTPSGSSE PALAGTSSSI LGPIASSTGL TEPEAKPPVP 1440
PSQSGNEGLL NLSSTAGTPS ADGIDEELLA SLAGE 1475 
Gene Ontology
 GO:0016592; C:mediator complex; IC:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IMP:UniProtKB.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:FlyBase. 
Interpro
 IPR019680; Mediator_Med1_met/fun. 
Pfam
 PF10744; Med1 
SMART
  
PROSITE
  
PRINTS