CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011423
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GA-binding protein alpha chain 
Protein Synonyms/Alias
 GABP subunit alpha; Nuclear respiratory factor 2 subunit alpha; Transcription factor E4TF1-60 
Gene Name
 GABPA 
Gene Synonyms/Alias
 E4TF1A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
359QPELVAQKWGQRKNKubiquitination[1, 2, 3, 4]
364AQKWGQRKNKPTMNYacetylation[5]
373KPTMNYEKLSRALRYacetylation[5]
452TASLQTEKDN*****ubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. 
Sequence Annotation
 DOMAIN 168 251 PNT.
 DNA_BIND 320 400 ETS.  
Keyword
 Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 454 AA 
Protein Sequence
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYAPAECVSQ AIDINEPIGN LKKLLEPRLQ 60
CSLDAHEICL QDIQLDPERS LFDQGVKTDG TVQLSVQVIS YQGIEPKLNI LEIVKPADTV 120
EVVIDPDAHH AESEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL 180
GIPYDPIQWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL 240
WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAP 300
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW 360
GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVTE 420
CEQKKLAKMQ LHGIAQPVTA VALATASLQT EKDN 454 
Gene Ontology
 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0046982; F:protein heterodimerization activity; IDA:MGI.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0001228; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:BHF-UCL.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara. 
Interpro
 IPR000418; Ets_dom.
 IPR024668; GABP_asu_N.
 IPR003118; Pointed_dom.
 IPR013761; SAM/pointed.
 IPR016312; TF_GA-bd_asu.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00178; Ets
 PF11620; GABP-alpha
 PF02198; SAM_PNT 
SMART
 SM00413; ETS
 SM00251; SAM_PNT 
PROSITE
 PS00345; ETS_DOMAIN_1
 PS00346; ETS_DOMAIN_2
 PS50061; ETS_DOMAIN_3
 PS51433; PNT 
PRINTS
 PR00454; ETSDOMAIN.