| Tag | Content |
|---|
| CPLM ID | CPLM-006176 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1 |
Protein Synonyms/Alias | ARS consensus-binding protein ACBP-60; Poly uridylate-binding protein; Poly(U)-binding protein |
Gene Name | PUB1 |
Gene Synonyms/Alias | RNP1; YNL016W; N2842 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 240 | LRINWAAKRDNNNNN | ubiquitination | [1] |
|
Reference | [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae. Starita LM, Lo RS, Eng JK, von Haller PD, Fields S. Proteomics. 2012 Jan;12(2):236-40. [ PMID: 22106047] |
Functional Description | May be associated with hnRNA within the nucleus and remains associated during nucleocytoplasmic mRNA transport, once the proteins are in the cytoplasm, disassembly of PUB1-RNA complexes may occur prior to PAB1 binding and formation of a translationally competent RNP complex. Binds to polyadenylated RNA; prefers to bind poly(rU); binds to T-rich single-stranded DNA. |
Sequence Annotation | DOMAIN 75 152 RRM 1.
DOMAIN 162 240 RRM 2.
DOMAIN 341 413 RRM 3.
REGION 260 264 RNA-binding RGG-box (By similarity).
MOD_RES 2 2 N-acetylserine. |
Keyword | 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Reference proteome; Repeat; RNA-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 453 AA |
Protein Sequence | MSENNEEQHQ QQQQQQPVAV ETPSAVEAPA SADPSSEQSV AVEGNSEQAE DNQGENDPSV 60
VPANAITGGR ETSDRVLYVG NLDKAITEDI LKQYFQVGGP IANIKIMIDK NNKNVNYAFV 120
EYHQSHDANI ALQTLNGKQI ENNIVKINWA FQSQQSSSDD TFNLFVGDLN VNVDDETLRN 180
AFKDFPSYLS GHVMWDMQTG SSRGYGFVSF TSQDDAQNAM DSMQGQDLNG RPLRINWAAK 240
RDNNNNNNYQ QRRNYGNNNR GGFRQYNSNN NNNMNMGMNM NMNMNMNNSR GMPPSSMGMP 300
IGAMPLPSQG QPQQSQTIGL PPQVNPQAVD HIIRSAPPRV TTAYIGNIPH FATEADLIPL 360
FQNFGFILDF KHYPEKGCCF IKYDTHEQAA VCIVALANFP FQGRNLRTGW GKERSNFMPQ 420
QQQQGGQPLI MNDQQQPVMS EQQQQQQQQQ QQQ 453 |
Gene Ontology | GO:0010494; C:cytoplasmic stress granule; IDA:SGD. GO:0005634; C:nucleus; IDA:SGD. GO:0003729; F:mRNA binding; IDA:SGD. GO:0000166; F:nucleotide binding; IEA:InterPro. GO:0008266; F:poly(U) RNA binding; IDA:SGD. GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:SGD. GO:0043488; P:regulation of mRNA stability; IMP:SGD. GO:0034063; P:stress granule assembly; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |