| Tag | Content |
|---|
| CPLM ID | CPLM-007960 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 6-phosphofructokinase, muscle type |
Protein Synonyms/Alias | Phosphofructo-1-kinase isozyme A; PFK-A; Phosphofructokinase-M; Phosphofructokinase 1; Phosphohexokinase |
Gene Name | Pfkm |
Gene Synonyms/Alias | Pfk-m; Pfka |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 141 | DLLNDLQKDGKITAE | ubiquitination | [1] | | 372 | KRFDEAIKLRGRSFM | ubiquitination | [1] | | 678 | FDRNFATKMGAKAMN | phosphoglycerylation | [2] | | 678 | FDRNFATKMGAKAMN | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] [2] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite. Moellering RE, Cravatt BF. Science. 2013 Aug 2;341(6145):549-53. [ PMID: 23908237] |
Functional Description | Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP. |
Sequence Annotation | NP_BIND 35 39 ATP (By similarity).
NP_BIND 193 197 ATP (By similarity).
NP_BIND 210 226 ATP (By similarity).
ACT_SITE 166 166 Proton acceptor (By similarity).
METAL 224 224 Magnesium; via carbonyl oxygen (By
BINDING 201 201 Substrate (By similarity).
BINDING 292 292 Substrate (By similarity).
BINDING 298 298 Substrate (By similarity).
BINDING 301 301 Substrate (By similarity).
MOD_RES 2 2 N-acetylthreonine (By similarity).
MOD_RES 667 667 Phosphoserine (By similarity).
MOD_RES 775 775 Phosphoserine (By similarity).
CARBOHYD 530 530 O-linked (GlcNAc...) (By similarity). |
Keyword | Acetylation; Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Glycoprotein; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 780 AA |
Protein Sequence | MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD 60
GGEHIREATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAHNLVKRGI TNLCVIGGDG 120
SLTGADTFRS EWSDLLNDLQ KDGKITAEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS 180
ALHRIVEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE 240
EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ 300
RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK 360
AMDEKRFDEA IKLRGRSFMN NWEVYKLLAH VRPPVSKGGL HTVAVMNVGA PAAGMNAAVR 420
STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKNLEQ 480
ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSI 540
GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF 600
TIRDLQVNVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG 660
HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF 720
QPVTELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI SRKRSGEAAV 780 |
Gene Ontology | GO:0005945; C:6-phosphofructokinase complex; IEA:InterPro. GO:0016324; C:apical plasma membrane; IEA:Compara. GO:0005829; C:cytosol; IDA:MGI. GO:0003872; F:6-phosphofructokinase activity; ISS:UniProtKB. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0070061; F:fructose binding; IEA:Compara. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0008443; F:phosphofructokinase activity; IDA:MGI. GO:0006002; P:fructose 6-phosphate metabolic process; IEA:InterPro. GO:0042593; P:glucose homeostasis; IMP:MGI. GO:0006096; P:glycolysis; IDA:MGI. GO:0046716; P:muscle cell homeostasis; IEA:Compara. GO:0032024; P:positive regulation of insulin secretion; IMP:MGI. GO:0051259; P:protein oligomerization; IEA:Compara. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |