CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012736
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADP-dependent malic enzyme, mitochondrial 
Protein Synonyms/Alias
 NADP-ME; Malic enzyme 3 
Gene Name
 ME3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
119TLQDRNEKLFYRVLTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
 ACT_SITE 137 137 Proton donor (By similarity).
 ACT_SITE 208 208 Proton acceptor (By similarity).
 METAL 280 280 Divalent metal cation (By similarity).
 METAL 281 281 Divalent metal cation (By similarity).
 METAL 304 304 Divalent metal cation (By similarity).
 BINDING 190 190 NAD (By similarity).
 BINDING 304 304 NAD (By similarity).
 BINDING 443 443 NAD (By similarity).  
Keyword
 Complete proteome; Metal-binding; Mitochondrion; NADP; Oxidoreductase; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 604 AA 
Protein Sequence
MGAALGTGTR LAPWPGRACG ALPRWTPTAP AQGCHSKPGP ARPVPLKKRG YDVTRNPHLN 60
KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYERQ QSDLDKYIIL MTLQDRNEKL 120
FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHLAT MLNSWPEDNI 180
KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD 240
PLYIGLKHQR VHGKAYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM 300
FNDDIQGTAS VAVAGILAAL RITKNKLSNH VFVFQGAGEA AMGIAHLLVM ALEKEGVPKA 360
EATRKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA 420
FTEQILRDMA SFHERPIIFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGK 480
TFIPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST 540
IRDVSLRIAI KVLDYAYKHN LASYYPEPKD KEAFVRSLVY TPDYDSFTLD SYTWPKEAMN 600
VQTV 604 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; TAS:UniProtKB.
 GO:0048037; F:cofactor binding; TAS:UniProtKB.
 GO:0004473; F:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; IDA:UniProtKB.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0009060; P:aerobic respiration; TAS:UniProtKB.
 GO:0006108; P:malate metabolic process; IDA:UniProtKB.
 GO:0072592; P:oxygen metabolic process; TAS:UniProtKB.
 GO:0006090; P:pyruvate metabolic process; IDA:UniProtKB. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.