CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002457
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 C-1-tetrahydrofolate synthase, cytoplasmic 
Protein Synonyms/Alias
 C1-THF synthase; Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase; Formyltetrahydrofolate synthetase 
Gene Name
 ADE3 
Gene Synonyms/Alias
 YGR204W; G7733 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
251NYVADPSKKSGFKCVacetylation[1]
297QNTLIAAKRQMEESSubiquitination[2]
362YKAKISPKVIERLQTubiquitination[2]
374LQTRQNGKYILVSGIubiquitination[2]
407ALTAHLGKPAIANVRubiquitination[2]
522LTPEEINKFARLNIDacetylation[1]
522LTPEEINKFARLNIDubiquitination[2]
558IGQAPTEKNHTRVTGacetylation[1]
662IADRVALKLVGTEPEubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 NP_BIND 169 171 NADP (By similarity).
 NP_BIND 384 391 ATP (By similarity).
 REGION 1 319 Methylenetetrahydrofolate dehydrogenase
 REGION 51 55 Substrate binding (By similarity).
 REGION 98 100 Substrate binding (By similarity).
 REGION 277 281 Substrate binding (By similarity).
 REGION 320 946 Formyltetrahydrofolate synthetase.
 BINDING 194 194 NADP (By similarity).
 MOD_RES 176 176 Phosphoserine.
 MOD_RES 318 318 Phosphothreonine.
 MOD_RES 322 322 Phosphoserine.  
Keyword
 Amino-acid biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Histidine biosynthesis; Hydrolase; Ligase; Methionine biosynthesis; Multifunctional enzyme; NADP; Nucleotide-binding; Nucleus; One-carbon metabolism; Oxidoreductase; Phosphoprotein; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 946 AA 
Protein Sequence
MAGQVLDGKA CAQQFRSNIA NEIKSIQGHV PGFAPNLAII QVGNRPDSAT YVRMKRKAAE 60
EAGIVANFIH LDESATEFEV LRYVDQLNED PHTHGIIVQL PLPAHLDEDR ITSRVLAEKD 120
VDGFGPTNIG ELNKKNGHPF FLPCTPKGII ELLHKANVTI EGSRSVVIGR SDIVGSPVAE 180
LLKSLNSTVT ITHSKTRDIA SYLHDADIVV VAIGQPEFVK GEWFKPRDGT SSDKKTVVID 240
VGTNYVADPS KKSGFKCVGD VEFNEAIKYV HLITPVPGGV GPMTVAMLMQ NTLIAAKRQM 300
EESSKPLQIP PLPLKLLTPV PSDIDISRAQ QPKLINQLAQ ELGIYSHELE LYGHYKAKIS 360
PKVIERLQTR QNGKYILVSG ITPTPLGEGK STTTMGLVQA LTAHLGKPAI ANVRQPSLGP 420
TLGVKGGAAG GGYSQVIPMD EFNLHLTGDI HAIGAANNLL AAAIDTRMFH ETTQKNDATF 480
YNRLVPRKNG KRKFTPSMQR RLNRLGIQKT NPDDLTPEEI NKFARLNIDP DTITIKRVVD 540
INDRMLRQIT IGQAPTEKNH TRVTGFDITV ASELMAILAL SKDLRDMKER IGRVVVAADV 600
NRSPVTVEDV GCTGALTALL RDAIKPNLMQ TLEGTPVLVH AGPFANISIG ASSVIADRVA 660
LKLVGTEPEA KTEAGYVVTE AGFDFTMGGE RFFNIKCRSS GLTPNAVVLV ATVRALKSHG 720
GAPDVKPGQP LPSAYTEENI EFVEKGAANM CKQIANIKQF GVPVVVAINK FETDTEGEIA 780
AIRKAALEAG AFEAVTSNHW AEGGKGAIDL AKAVIEASNQ PVDFHFLYDV NSSVEDKLTT 840
IVQKMYGGAA IDILPEAQRK IDMYKEQGFG NLPICIAKTQ YSLSHDATLK GVPTGFTFPI 900
RDVRLSNGAG YLYALAAEIQ TIPGLATYAG YMAVEVDDDG EIDGLF 946 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004329; F:formate-tetrahydrofolate ligase activity; IMP:SGD.
 GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IMP:SGD.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IMP:SGD.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
 GO:0006760; P:folic acid-containing compound metabolic process; IMP:SGD.
 GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009113; P:purine nucleobase biosynthetic process; IMP:SGD.
 GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 
Interpro
 IPR000559; Formate_THF_ligase.
 IPR020628; Formate_THF_ligase_CS.
 IPR016040; NAD(P)-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020867; THF_DH/CycHdrlase_CS.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 
Pfam
 PF01268; FTHFS
 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 
SMART
  
PROSITE
 PS00721; FTHFS_1
 PS00722; FTHFS_2
 PS00766; THF_DHG_CYH_1
 PS00767; THF_DHG_CYH_2 
PRINTS
 PR00085; THFDHDRGNASE.