CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032079
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fatty acid desaturase 2 
Protein Synonyms/Alias
 cDNA FLJ56373, highly similar to Homo sapiens fatty acid desaturase 2 (FADS2), mRNA 
Gene Name
 FADS2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53PDLEFVGKFLKPLLIubiquitination[1, 2, 3]
56EFVGKFLKPLLIGELubiquitination[1, 2, 3, 4, 5, 6]
74EPSQDHGKNSKITEDubiquitination[2, 3, 5]
77QDHGKNSKITEDFRAubiquitination[2, 3, 4, 5, 6]
87EDFRALRKTAEDMNLubiquitination[2, 3, 6]
161LSVYRKPKWNHLVHKubiquitination[2]
168KWNHLVHKFVIGHLKubiquitination[2]
175KFVIGHLKGASANWWubiquitination[1, 2, 6]
192RHFQHHAKPNIFHKDubiquitination[2, 4]
220WQPIEYGKKKLKYLPubiquitination[3]
367MPRHNLHKIAPLVKSubiquitination[1, 2, 3, 4, 5, 6]
373HKIAPLVKSLCAKHGubiquitination[1, 2, 5, 6]
378LVKSLCAKHGIEYQEubiquitination[2, 5, 6]
386HGIEYQEKPLLRALLubiquitination[1, 2, 3, 4, 5, 6]
401DIIRSLKKSGKLWLDubiquitination[2]
404RSLKKSGKLWLDAYLubiquitination[1, 2, 3, 5, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 413 AA 
Protein Sequence
MTREPPGCRR VNSLMLYTLR SITSHRSSHP ERWATSSQDA FRAFHPDLEF VGKFLKPLLI 60
GELAPEEPSQ DHGKNSKITE DFRALRKTAE DMNLFKTNHV FFLLLLAHII ALESIAWFTV 120
FYFGNGWIPT LITAFVLATS QAQAGWLQHD YGHLSVYRKP KWNHLVHKFV IGHLKGASAN 180
WWNHRHFQHH AKPNIFHKDP DVNMLHVFVL GEWQPIEYGK KKLKYLPYNH QHEYFFLIGP 240
PLLIPMYFQY QIIMTMIVHK NWVDLAWAVS YYIRFFITYI PFYGILGALL FLNFIRFLES 300
HWFVWVTQMN HIVMEIDQEA YRDWFSSQLT ATCNVEQSFF NDWFSGHLNF QIEHHLFPTM 360
PRHNLHKIAP LVKSLCAKHG IEYQEKPLLR ALLDIIRSLK KSGKLWLDAY LHK 413 
Gene Ontology
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0016717; F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; IEA:InterPro.
 GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro. 
Interpro
 IPR012171; Fatty_acid/sphinglp_desaturase.
 IPR005804; Fatty_acid_desaturase-1. 
Pfam
 PF00487; FA_desaturase 
SMART
  
PROSITE
  
PRINTS