UniProt Accession

 HCDH_RAT; Q9WVK7 

Genbank Protein ID

 AF095449 

Genbank Nucleotide ID

 AAD42162.1 

Protein Name

 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial 

Protein Synonyms/Alias

 HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase 

Gene Name

 Hadh 

Gene Synonyms/Alias

 Had; Hadhsc; Schad 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
67	EDILAKSKKGIEESL	acetylation	[1]
68	DILAKSKKGIEESLK	acetylation	[1]
75	KGIEESLKRMAKKKF	acetylation	[1]
80	SLKRMAKKKFTENPK	acetylation	[1]
81	LKRMAKKKFTENPKA	acetylation	[1]
87	KKFTENPKAADEFVE	acetylation	[1]
125	EAIVENLKLKNELFQ	acetylation	[1]
136	ELFQRLDKFAAEHTI	acetylation	[1]
179	FNPVPMMKLVEVIKT	acetylation	[1]
185	MKLVEVIKTPMTSQK	acetylation	[1]
192	KTPMTSQKTFESLVD	acetylation	[1]
202	ESLVDFCKTLGKHPV	acetylation	[1]
206	DFCKTLGKHPVSCKD	acetylation	[1]
212	GKHPVSCKDTPGFIV	acetylation	[1]
241	HERGDASKEDIDTAM	acetylation	[1]
249	EDIDTAMKLGAGYPM	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Plays an essential role in the mitochondrial beta- oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. 

Sequence Annotation

 NP_BIND 34 39 NAD (By similarity).
 BINDING 57 57 NAD (By similarity).
 BINDING 73 73 Coenzyme A (By similarity).
 BINDING 80 80 Coenzyme A (By similarity).
 BINDING 122 122 NAD (By similarity).
 BINDING 127 127 NAD (By similarity).
 BINDING 149 149 Coenzyme A (By similarity).
 BINDING 149 149 NAD (By similarity).
 BINDING 173 173 NAD (By similarity).
 BINDING 305 305 NAD (By similarity).
 MOD_RES 185 185 N6-acetyllysine (By similarity).
 MOD_RES 202 202 N6-acetyllysine (By similarity).
 MOD_RES 241 241 N6-acetyllysine (By similarity).
 MOD_RES 312 312 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Fatty acid metabolism; Lipid metabolism; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 314 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IMP:RGD.
 GO:0070403; F:NAD+ binding; IEA:InterPro.
 GO:0006635; P:fatty acid beta-oxidation; IMP:RGD.
 GO:0046676; P:negative regulation of insulin secretion; IMP:RGD.
 GO:0014823; P:response to activity; IDA:RGD.
 GO:0042493; P:response to drug; IDA:RGD.
 GO:0032868; P:response to insulin stimulus; IEP:RGD. 

Interpro

 IPR022694; 3-OHacyl-CoA_DH.
 IPR006180; 3-OHacyl-CoA_DH_CS.
 IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
 IPR006108; 3HC_DH_C.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00725; 3HCDH
 PF02737; 3HCDH_N 

SMART

  

PROSITE

 PS00067; 3HCDH 

PRINTS