UniProt Accession

 PREA_ECOLI; P25889; P76441; Q2MAT2 

Genbank Protein ID

 U00096; AP009048; M59444 

Genbank Nucleotide ID

 AAC75208.2; BAE76624.1 

Protein Name

 NAD-dependent dihydropyrimidine dehydrogenase subunit PreA 

Protein Synonyms/Alias

 DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase 

Gene Name

 preA 

Gene Synonyms/Alias

 yeiA; b2147; JW2134 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
377	TPHCNTEKCVGCLLC	acetylation	[1]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Involved in pyrimidine base degradation. Catalyzes physiologically the reduction of uracil to 5,6-dihydrouracil (DHU) by using NADH as a specific cosubstrate. It also catalyzes the reverse reaction and the reduction of thymine to 5,6- dihydrothymine (DHT). 

Sequence Annotation

 DOMAIN 335 367 4Fe-4S ferredoxin-type 1.
 DOMAIN 369 398 4Fe-4S ferredoxin-type 2.
 REGION 134 136 Substrate binding (By similarity).
 REGION 201 202 Substrate binding (By similarity).
 ACT_SITE 137 137 Proton acceptor (By similarity).
 METAL 344 344 Iron-sulfur 1 (4Fe-4S) (Potential).
 METAL 347 347 Iron-sulfur 1 (4Fe-4S) (Potential).
 METAL 350 350 Iron-sulfur 1 (4Fe-4S) (Potential).
 METAL 354 354 Iron-sulfur 2 (4Fe-4S) (Potential).
 METAL 378 378 Iron-sulfur 2 (4Fe-4S) (Potential).
 METAL 381 381 Iron-sulfur 2 (4Fe-4S) (Potential).
 METAL 384 384 Iron-sulfur 2 (4Fe-4S) (Potential).
 METAL 388 388 Iron-sulfur 1 (4Fe-4S) (Potential).
 BINDING 76 76 Substrate (By similarity).  

Keyword

 4Fe-4S; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 411 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0004158; F:dihydroorotate oxidase activity; IEA:InterPro.
 GO:0004159; F:dihydrouracil dehydrogenase (NAD+) activity; IEA:EC.
 GO:0051536; F:iron-sulfur cluster binding; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003954; F:NADH dehydrogenase activity; IDA:UniProtKB.
 GO:0006928; P:cellular component movement; IMP:EcoCyc.
 GO:0006208; P:pyrimidine nucleobase catabolic process; IDA:UniProtKB.
 GO:0006222; P:UMP biosynthetic process; IEA:InterPro. 

Interpro

 IPR017896; 4Fe4S_Fe-S-bd.
 IPR017900; 4Fe4S_Fe_S_CS.
 IPR013785; Aldolase_TIM.
 IPR005720; Dihydroorotate_DH.
 IPR012135; Dihydroorotate_DH_1_2. 

Pfam

 PF01180; DHO_dh 

SMART

  

PROSITE

 PS00198; 4FE4S_FER_1
 PS51379; 4FE4S_FER_2 

PRINTS