CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039932
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Protein HIRA 
Protein Synonyms/Alias
  
Gene Name
 HIRA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
70TVFGSSGKLANVEQWacetylation[1, 2]
70TVFGSSGKLANVEQWubiquitination[2, 3]
134RGHSGLVKGLTWDPVubiquitination[2, 3, 4]
143LTWDPVGKYIASQADubiquitination[2, 3, 5]
170QLETSITKPFDECGGubiquitination[2, 3]
215IIEREGWKTNMDFVGubiquitination[3]
231RKAVTVVKFNPKIFKubiquitination[2, 3]
235TVVKFNPKIFKKKQKubiquitination[3]
248QKNGSSAKPSCPYCCubiquitination[3]
337IHQSTYGKSLAIMTEubiquitination[3]
372QQQQLDQKSAATREMubiquitination[2, 3]
556LKEQNLVKELRPRDLacetylation[6]
684LKCNREGKEWETVLTubiquitination[3]
710VVCVACEKRMLSVFSubiquitination[3]
816TWNLVSDKQDSLAQCubiquitination[3]
944LRKRELLKELLPVIGubiquitination[3]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 973 AA 
Protein Sequence
MSPVLQEDDE KDENIPKMLC QMDNHLACVN CVRWSNSGMY LASGGDDKLI MVWKRATYIG 60
PSTVFGSSGK LANVEQWRCV SILRNHSGDV MDVAWSPHDA WLASCSVDNT VVIWNAVKFP 120
EILATLRGHS GLVKGLTWDP VGKYIASQAD DRSLKVWRTL DWQLETSITK PFDECGGTTH 180
VLRLSWSPDG HYLVSAHAMN NSGPTAQIIE REGWKTNMDF VGHRKAVTVV KFNPKIFKKK 240
QKNGSSAKPS CPYCCCAVGS KDRSLSVWLT CLKRPLVVIH ELFDKSIMDI SWTLNGLGIL 300
VCSMDGSVAF LDFSQDELGD PLSEEEKSRI HQSTYGKSLA IMTEAQLSTA VIENPEMLKY 360
QRRQQQQQLD QKSAATREMG SATSVAGVVN GESLEDIRKN LLKKQVETRT ADGRRRITPL 420
CIAQLDTGDF STAFFNSIPL SGSLAGTMLS SHSSPQLLPL DSSTPNSFGA SKPCTEPVVA 480
ASARPAGDSV NKDSMNATST PAALSPSVLT TPSKIEPMKA FDSRFTERSK ATPGAPALTS 540
MTPTAVERLK EQNLVKELRP RDLLESSSDS DEKVPLAKAS SLSKRKLELE VETVEKKKKG 600
RPRKDSRLMP VSLSVQSPAA LTAEKEAMCL SAPALALKLP IPSPQRAFTL QVSSDPSMYI 660
EVENEVTVVG GVKLSRLKCN REGKEWETVL TSRILTAAGS CDVVCVACEK RMLSVFSTCG 720
RRLLSPILLP SPISTLHCTG SYVMALTAAA TLSVWDVHRQ VVVVKEESLH SILAGSDMTV 780
SQILLTQHGI PVMNLSDGKA YCFNPSLSTW NLVSDKQDSL AQCADFRSSL PSQDAMLCSG 840
PLAIIQGRTS NSGRQAARLF SVPHVVQQET TLAYLENQVA AALTLQSSHE YRHWLLVYAR 900
YLVNEGFEYR LREICKDLLG PVHYSTGSQW ESTVVGLRKR ELLKELLPVI GQNLRFQRLF 960
TECQEQLDIL RDK 973 
Gene Ontology
 GO:0000790; C:nuclear chromatin; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0016568; P:chromatin modification; IEA:InterPro.
 GO:0007369; P:gastrulation; IEA:Compara.
 GO:0042692; P:muscle cell differentiation; IEA:Compara.
 GO:0001649; P:osteoblast differentiation; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro. 
Interpro
 IPR011494; Hira.
 IPR019015; HIRA_B_motif.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF07569; Hira
 PF09453; HIRA_B
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS