CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033271
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Phosphoribosylformylglycinamidine cyclo-ligase 
Protein Synonyms/Alias
  
Gene Name
 GART 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20REHTLAWKLAQSHHVubiquitination[1]
28LAQSHHVKQVLVAPGacetylation[2]
28LAQSHHVKQVLVAPGubiquitination[1, 2, 3, 4]
44AGTACSEKISNTAISubiquitination[1]
63TALAQFCKEKKIEFVubiquitination[1]
65LAQFCKEKKIEFVVVubiquitination[1]
66AQFCKEKKIEFVVVGubiquitination[1, 5, 6]
107AAQLESSKRFAKEFMubiquitination[1, 2, 3, 4, 5, 7]
111ESSKRFAKEFMDRHGubiquitination[1, 2, 4, 5]
129AQWKAFTKPEEACSFubiquitination[1]
148DFPALVVKASGLAAGubiquitination[1]
156ASGLAAGKGVIVAKSubiquitination[1, 4, 5, 7]
162GKGVIVAKSKEEACKubiquitination[1, 4]
169KSKEEACKAVQEIMQubiquitination[1]
219MPPAQDHKRLLEGDGubiquitination[1, 5, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 232 AA 
Protein Sequence
MAARVLIIGS GGREHTLAWK LAQSHHVKQV LVAPGNAGTA CSEKISNTAI SISDHTALAQ 60
FCKEKKIEFV VVGPEAPLAA GIVGNLRSAG VQCFGPTAEA AQLESSKRFA KEFMDRHGIP 120
TAQWKAFTKP EEACSFILSA DFPALVVKAS GLAAGKGVIV AKSKEEACKA VQEIMQEKAF 180
GAAGETIVIE ELLDGEEVSC LCFTDGKTVA PMPPAQDHKR LLEGDGGPNT GG 232 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:InterPro.
 GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro. 
Interpro
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR016185; PreATP-grasp_dom.
 IPR020561; PRibGlycinamid_synth_ATP-grasp.
 IPR000115; PRibGlycinamide_synth.
 IPR020562; PRibGlycinamide_synth_N. 
Pfam
 PF01071; GARS_A
 PF02844; GARS_N 
SMART
  
PROSITE
 PS50975; ATP_GRASP 
PRINTS