CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001816
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Diaminopimelate decarboxylase 
Protein Synonyms/Alias
 DAP decarboxylase; DAPDC 
Gene Name
 lysA 
Gene Synonyms/Alias
 b2838; JW2806 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
44RRQIAALKQFDVVRFacetylation[1]
264RHLGHPVKLEIEPGRacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine. Is not active against the DD- or LL-isomers of diaminopimelate. 
Sequence Annotation
 REGION 268 271 Pyridoxal phosphate binding.
 ACT_SITE 342 342 Proton donor (Potential).
 BINDING 191 191 Substrate.
 BINDING 227 227 Pyridoxal phosphate; via amide nitrogen.
 BINDING 271 271 Substrate.
 BINDING 307 307 Substrate.
 BINDING 311 311 Substrate.
 BINDING 343 343 Substrate.
 BINDING 378 378 Pyridoxal phosphate.
 BINDING 378 378 Substrate.
 BINDING 382 382 Substrate.
 MOD_RES 54 54 N6-(pyridoxal phosphate)lysine.  
Keyword
 3D-structure; Amino-acid biosynthesis; Complete proteome; Decarboxylase; Lyase; Lysine biosynthesis; Pyridoxal phosphate; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 420 AA 
Protein Sequence
MPHSLFSTDT DLTAENLLRL PAEFGCPVWV YDAQIIRRQI AALKQFDVVR FAQKACSNIH 60
ILRLMREQGV KVDSVSLGEI ERALAAGYNP QTHPDDIVFT ADVIDQATLE RVSELQIPVN 120
AGSVDMLDQL GQVSPGHRVW LRVNPGFGHG HSQKTNTGGE NSKHGIWYTD LPAALDVIQR 180
HHLQLVGIHM HIGSGVDYAH LEQVCGAMVR QVIEFGQDLQ AISAGGGLSV PYQQGEEAVD 240
TEHYYGLWNA AREQIARHLG HPVKLEIEPG RFLVAQSGVL ITQVRSVKQM GSRHFVLVDA 300
GFNDLMRPAM YGSYHHISAL AADGRSLEHA PTVETVVAGP LCESGDVFTQ QEGGNVETRA 360
LPEVKAGDYL VLHDTGAYGA SMSSNYNSRP LLPEVLFDNG QARLIRRRQT IEELLALELL 420 
Gene Ontology
 GO:0008836; F:diaminopimelate decarboxylase activity; IDA:EcoCyc.
 GO:0030170; F:pyridoxal phosphate binding; IEA:HAMAP.
 GO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:HAMAP. 
Interpro
 IPR009006; Ala_racemase/Decarboxylase_C.
 IPR002986; DAP_deCOOHase_LysA.
 IPR022643; De-COase2_C.
 IPR022657; De-COase2_CS.
 IPR022644; De-COase2_N.
 IPR022653; De-COase2_pyr-phos_BS.
 IPR000183; Orn/DAP/Arg_de-COase. 
Pfam
 PF02784; Orn_Arg_deC_N
 PF00278; Orn_DAP_Arg_deC 
SMART
  
PROSITE
 PS00878; ODR_DC_2_1
 PS00879; ODR_DC_2_2 
PRINTS
 PR01181; DAPDCRBXLASE.
 PR01179; ODADCRBXLASE.