UniProt Accession

 DPH2_HUMAN; Q9BQC3; A8MVC9; B2RDE3; O60623 

Genbank Protein ID

 AF053003; AK315506; BT007431; AL357079; CH471059; BC001389; BC003181; BC016956 

Genbank Nucleotide ID

 AAC18086.1; BAG37890.1; AAP36099.1; CAI16800.1; EAX07070.1; AAH01389.1; AAH03181.1; AAH16956.1 

Protein Name

 Diphthamide biosynthesis protein 2 

Protein Synonyms/Alias

 DPH2 homolog; HsDph2; Diphthamide biosynthesis protein 2 homolog-like 2; DPH2-like 2 

Gene Name

 DPH2 

Gene Synonyms/Alias

 DPH2L2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
146	EAQNPDPKAPVVLLS	ubiquitination	[1]
223	AFYVGGSKASPDPDL	ubiquitination	[1, 2, 3]
256	SCCPDTGKTQDEGAR	ubiquitination	[1, 3]
312	NLTQAAGKRSYVLAL	ubiquitination	[1]
424	LRPPPAWKSSNDHGS	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (By similarity). 

Sequence Annotation

 MOD_RES 435 435 Phosphothreonine.
 MOD_RES 446 446 Phosphoserine.
 MOD_RES 467 467 Phosphothreonine.
 MOD_RES 488 488 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 489 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IEA:InterPro. 

Interpro

 IPR010014; DHP2_eu.
 IPR002728; DPH1/DPH2. 

Pfam

 PF01866; Diphthamide_syn 

SMART

  

PROSITE

  

PRINTS