CPLM-016376

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-016376

UniProt Accession

MIRO1_MOUSE; Q8BG51; Q3TJB7; Q3UGU4; Q5SYC3; Q5SYC6; Q8BLW3; Q8BMH1; Q922N0; Q9D2R1; Q9JKB9

Genbank Protein ID

AK019059; AK028597; AK031139; AK041119; AK054472; AK078955; AK138830; AK141621; AK147749; AK147801; AK167502; AL591426; AL591426; AL591426; AL591426; BC006918; BC046785; BC058350; AF244542

Genbank Nucleotide ID

BAB31529.2; BAC26025.1; BAC27274.1; BAC30828.1; BAC35792.1; BAC37478.1; BAE23792.1; BAE24771.1; BAE28113.1; BAE28148.1; BAE39578.1; CAI25293.1; CAI25294.1; CAI25295.1; CAI25296.1; AAH06918.1; AAH46785.1; AAH58350.1; AAF64187.1

Protein Name

Mitochondrial Rho GTPase 1

Protein Synonyms/Alias

MIRO-1; Ras homolog gene family member T1

Gene Name

Rhot1

Gene Synonyms/Alias

Arht1

Created Date

July 27, 2013

Organism

Mus musculus (Mouse)

NCBI Taxa ID

10090

Lysine Modification

Position	Peptide	Type	References
166	ECSAKNLKNISELFY	ubiquitination	[1]
195	CPEEKEMKPACIKAL	ubiquitination	[1]
200	EMKPACIKALTRIFK	ubiquitination	[1]
207	KALTRIFKISDQDND	ubiquitination	[1]

Reference

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity).

Sequence Annotation

DOMAIN 11 190 Miro 1.
DOMAIN 197 232 EF-hand 1.
DOMAIN 317 352 EF-hand 2.
DOMAIN 425 574 Miro 2.
NP_BIND 24 31 GTP 1 (Potential).
NP_BIND 70 74 GTP 1 (Potential).
NP_BIND 131 134 GTP 1 (Potential).
NP_BIND 438 445 GTP 2 (Potential).
NP_BIND 476 480 GTP 2 (Potential).
NP_BIND 540 543 GTP 2 (Potential).

Keyword

Alternative splicing; Calcium; Complete proteome; GTP-binding; Hydrolase; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Reference proteome; Repeat; Transmembrane; Transmembrane helix.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

631 AA

Protein Sequence

MRAGRVRPLR ASDMKKDVRI LLVGEPRVGK TSLIMSLVSE EFPEEVPPRA EEITIPADVT 60
PERVPTHIVD YSEAEQSDEQ LHQEISQANV ICIVYAVNNK HSIDKVTSRW IPLINERTDK 120
DSRLPLILVG NKSDLVEYSS METILPIMNQ YTEIETCVEC SAKNLKNISE LFYYAQKAVL 180
HPTGPLYCPE EKEMKPACIK ALTRIFKISD QDNDGTLNDA ELNFFQRICF NTPLAPQALE 240
DVKNVVRKHL SDGVADSGLT LRGFLFLHTL FIQRGRHETT WTVLRRFGYD DDLDLTPEYL 300
FPLLKIPPDC TTELNHHAYL FLQSTFDKHD LDRDCALSPD ELKDLFQVFP YIPWGPDVNN 360
TVCTNERGWI TYQGFLSQWT LTTYLDVQRC LEYLGYLGYS ILTEQESQAS AITVTRDKKI 420
DLQKKQTQRN VFRCNVIGVK GCGKTGVLQS LLGRNLMRQK KIRDDHKSYY AINTVYVYGQ 480
EKYLLLHDIS ESEFLTEAET ICDVVCLVYD VTNPKSFEYC ARIFKQHFMD SRIPCLIVAA 540
KSDLHEVKQE HSISPTDFCR KHKMPPPQAF TCNTADAPSK DIFVKLTTMA MYPHVTQADL 600
KSSTFWLRAS FGATVFAVVG FAMYRALLKQ R 631

Gene Ontology

GO:0031307; C:integral to mitochondrial outer membrane; ISS:UniProtKB.
GO:0005509; F:calcium ion binding; IEA:InterPro.
GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO:0003924; F:GTPase activity; IEA:InterPro.
GO:0019725; P:cellular homeostasis; ISS:UniProtKB.
GO:0006184; P:GTP catabolic process; IEA:GOC.
GO:0097345; P:mitochondrial outer membrane permeabilization; ISS:UniProtKB.
GO:0047497; P:mitochondrion transport along microtubule; ISS:UniProtKB.
GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.

Interpro

IPR011992; EF-hand-like_dom.
IPR018247; EF_Hand_1_Ca_BS.
IPR013566; EF_hand_assoc_1.
IPR013567; EF_hand_assoc_2.
IPR002048; EF_hand_dom.
IPR020860; MIRO.
IPR013684; MIRO-like.
IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR021181; Small_GTPase_Miro.

Pfam

PF08355; EF_assoc_1
PF08356; EF_assoc_2
PF13405; EF_hand_4
PF08477; Miro
PF00071; Ras

SMART

SM00054; EFh

PROSITE

PS00018; EF_HAND_1
PS50222; EF_HAND_2
PS51423; MIRO

PRINTS

PR00449; RASTRNSFRMNG.