CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033508
UniProt Accession
Genbank Protein ID
 Z97056 
Genbank Nucleotide ID
  
Protein Name
 Probable ATP-dependent RNA helicase DDX17 
Protein Synonyms/Alias
  
Gene Name
 DDX17 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42GERLRKKKWDLSELPubiquitination[1, 2]
50WDLSELPKFEKNFYVacetylation[3]
50WDLSELPKFEKNFYVubiquitination[1, 2]
53SELPKFEKNFYVEHPubiquitination[1, 2, 4]
190QVADDYGKCSRLKSTubiquitination[2, 4, 5, 6]
234IDFLESGKTNLRRCTubiquitination[2, 4]
373AMCIHGDKSQPERDWubiquitination[2, 4]
389LNEFRSGKAPILIATubiquitination[2, 4]
438RTARSTNKGTAYTFFubiquitination[1, 2, 4]
451FFTPGNLKQARELIKubiquitination[1, 2, 4]
470ANQAINPKLMQLVDHubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 652 AA 
Protein Sequence
MRGGGFGDRD RDRDRGGFGA RGGGGLPPKK FGNPGERLRK KKWDLSELPK FEKNFYVEHP 60
EVARLTPYEV DELRRKKEIT VRGGDVCPKP VFAFHHANFP QYVMDVLMDQ HFTEPTPIQC 120
QGFPLALSGR DMVGIAQTGS GKTLAYLLPA IVHINHQPYL ERGDGPICLV LAPTRELAQQ 180
VQQVADDYGK CSRLKSTCIY GGAPKGPQIR DLERGVEICI ATPGRLIDFL ESGKTNLRRC 240
TYLVLDEADR MLDMGFEPQI RKIVDQIRPD RQTLMWSATW PKEVRQLAED FLRDYTQINV 300
GNLELSANHN ILQIVDVCME SEKDHKLIQL MEEIMAEKEN KTIIFVETKR RCDDLTRRMR 360
RDGWPAMCIH GDKSQPERDW VLNEFRSGKA PILIATDVAS RGLGFDVEDV KFVINYDYPN 420
SSEDYVHRIG RTARSTNKGT AYTFFTPGNL KQARELIKVL EEANQAINPK LMQLVDHRGG 480
GGGGGGRSRY RTTSSANNPN LMYQDECDRR LRGVKDGGRR DSASYRDRSE TDRAGYANGS 540
GYGSPNSAFG AQAGQYTYGQ GTYGAAAYGT SSYTAQEYGA GTYGASSTTS TGRSSQSSSQ 600
QFSGIGRSGQ QPQPLMSQQF AQPPGATNMI GYMGQTAYQY PPPPPPPPPS RK 652 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS