Tag | Content |
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CPLM ID | CPLM-000548 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ribosomal RNA small subunit methyltransferase NEP1 |
Protein Synonyms/Alias | 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1 |
Gene Name | Emg1 |
Gene Synonyms/Alias | C2f; Grcc2f |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
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59 | LETVKVGKTYELLNC | ubiquitination | [1] | 154 | RAADGPQKLLKVIKN | ubiquitination | [1] | 160 | QKLLKVIKNPVSDHF | ubiquitination | [1] |
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Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 1248 in human 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3- carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA (By similarity). |
Sequence Annotation | REGION 219 224 S-adenosyl-L-methionine binding (By BINDING 201 201 S-adenosyl-L-methionine; via amide BINDING 206 206 S-adenosyl-L-methionine; via carbonyl MOD_RES 16 16 Phosphoserine. |
Keyword | Complete proteome; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing; rRNA-binding; S-adenosyl-L-methionine; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 244 AA |
Protein Sequence | MSAASGGFQP RERRFSVQEQ DWETTPPKKL RLGAGSKCGG RRLIVVLEGA SLETVKVGKT 60 YELLNCDRHK SMLLKNGRDP GEVRPDITHQ SLLMLMDSPL NRAGLLQVYI HTQKNVLIEV 120 NPQTRIPRTF DRFCGLMVQL LHKLSVRAAD GPQKLLKVIK NPVSDHFPVG CMKIGTSFSV 180 EDISDIRELV PSSDPVVFVV GAFAHGKVSV EYTEKMVSIS NYPLSAALTC AKVTTAFEEV 240 WGVI 244 |
Gene Ontology | GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell. GO:0070037; F:rRNA (pseudouridine) methyltransferase activity; ISS:UniProtKB. GO:0019843; F:rRNA binding; IEA:UniProtKB-KW. |
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