UniProt Accession

 NDUS3_MOUSE; Q9DCT2; Q8BTZ3; Q8R073 

Genbank Protein ID

 AK002501; AK088337; AL672241; BC027270; BC119267; BC119269 

Genbank Nucleotide ID

 BAB22149.1; BAC40291.1; CAM15313.1; AAH27270.1; AAI19268.1; AAI19270.1 

Protein Name

 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial 

Protein Synonyms/Alias

 Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit 

Gene Name

 Ndufs3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
42	RESAAADKRPTVRPR	acetylation	[1, 2]
216	LRYDDEVKRVVAEPV	acetylation	[1]
252	RQPPESLKLEAGDKK	acetylation	[1]
258	LKLEAGDKKPETK**	acetylation	[1, 2]
259	KLEAGDKKPETK***	acetylation	[1, 3]
263	GDKKPETK*******	acetylation	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 

Functional Description

 Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). 

Sequence Annotation

  

Keyword

 Complete proteome; Direct protein sequencing; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Reference proteome; Respiratory chain; Transit peptide; Transport; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 263 AA 

Protein Sequence

Gene Ontology

 GO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:EC.
 GO:0003954; F:NADH dehydrogenase activity; ISS:UniProtKB.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0006917; P:induction of apoptosis; ISS:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; ISS:UniProtKB.
 GO:0072593; P:reactive oxygen species metabolic process; ISS:UniProtKB. 

Interpro

 IPR010218; NADH_DH_suC.
 IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
 IPR020396; NADH_UbQ_OxRdtase_CS. 

Pfam

 PF00329; Complex1_30kDa 

SMART

  

PROSITE

 PS00542; COMPLEX1_30K 

PRINTS