CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032187
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein-L-isoaspartate O-methyltransferase 
Protein Synonyms/Alias
  
Gene Name
 PCMT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MAWKSGGASHSubiquitination[1, 2, 3]
70RMVGCTGKVIGIDHIubiquitination[1]
139QALIDQLKPGGRLILubiquitination[4]
162QMLEQYDKLQDGSIKubiquitination[2, 5]
171QDGSIKMKPLMGVIYubiquitination[1, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 192 AA 
Protein Sequence
MAWKSGGASH SELIHNLRSF QATISAPHMH AYALELLFDQ LHEGAKALDV GSGSGILTAC 60
FARMVGCTGK VIGIDHIKEL VDDSVNNVRK DDPTLLSSGR VQLVVGDGRM GYAEEAPYDA 120
IHVGAAAPVV PQALIDQLKP GGRLILPVGP AGGNQMLEQY DKLQDGSIKM KPLMGVIYVP 180
LTDKEKQWSR WK 192 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0004719; F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity; IEA:EC. 
Interpro
 IPR000682; PCMT. 
Pfam
 PF01135; PCMT 
SMART
  
PROSITE
 PS01279; PCMT 
PRINTS