UniProt Accession

 NUP50_HUMAN; Q9UKX7; B1AHA4; B2RB15; O75644; Q8N6V5; Q9NPM9; Q9NPR6; Q9P1K5 

Genbank Protein ID

 AF107840; AF116624; CR456533; AK314454; AL008718; Z82243; Z82243; AL008718; Z82243; Z82243; AL008718; AL008718; Z82243; CH471138; CH471138; BC016055; BC028125; BC070133; AL389949; AL389950 

Genbank Nucleotide ID

 AAD53401.1; AAF71047.1; CAG30419.1; BAG37062.1; CAI17944.1; CAI17944.1; CAI17926.1; CAI17926.1; CAQ07372.1; CAQ07374.1; CAQ07374.1; CAQ08670.1; CAQ08670.1; EAW73370.1; EAW73372.1; AAH16055.1; AAH28125.1; AAH70133.1; CAB97527.1; CAB97528.1 

Protein Name

 Nuclear pore complex protein Nup50 

Protein Synonyms/Alias

 50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50 

Gene Name

 NUP50 

Gene Synonyms/Alias

 NPAP60L; PRO1146 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
55	FGSGAGGKPLEGLSN	ubiquitination	[1]
76	APPFASAKAAADPKV	ubiquitination	[1]
82	AKAAADPKVAFGSLA	ubiquitination	[1]
99	GPTTLVDKVSNPKTN	ubiquitination	[1]
164	PIFKDYEKYLANIEQ	ubiquitination	[1]
247	SASFNFGKKVDSSVL	ubiquitination	[1]
318	EENDEPPKVVVTEVK	ubiquitination	[1]
325	KVVVTEVKEEDAFYS	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. 

Sequence Annotation

 REPEAT 76 77 1.
 REPEAT 113 114 2.
 REPEAT 225 226 3.
 REPEAT 273 274 4.
 REPEAT 303 304 5.
 DOMAIN 335 468 RanBD1.
 REGION 76 304 5 X 2 AA repeats of F-G.
 REGION 144 206 Binding to CDKN1B (By similarity).
 MOD_RES 83 83 N6-acetyllysine.
 MOD_RES 115 115 Phosphoserine (By similarity).
 MOD_RES 221 221 Phosphoserine.
 MOD_RES 246 246 Phosphothreonine.
 MOD_RES 259 259 Phosphothreonine.
 MOD_RES 296 296 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 468 AA 

Protein Sequence

Gene Ontology

 GO:0031965; C:nuclear membrane; IDA:UniProtKB.
 GO:0005643; C:nuclear pore; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0046907; P:intracellular transport; IEA:InterPro.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR015007; NUP2/50/61.
 IPR011993; PH_like_dom.
 IPR000156; Ran_bind_dom. 

Pfam

 PF08911; NUP50
 PF00638; Ran_BP1 

SMART

 SM00160; RanBD 

PROSITE

 PS50196; RANBD1 

PRINTS