CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000911
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intron-binding protein aquarius 
Protein Synonyms/Alias
 Intron-binding protein of 160 kDa; IBP160 
Gene Name
 AQR 
Gene Synonyms/Alias
 KIAA0560 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAAPAQPKKIVAPTVubiquitination[1]
9AAPAQPKKIVAPTVSubiquitination[1]
52VIEDIYEKEIVKSRFubiquitination[1]
188ARLELELKKTPKLRKubiquitination[1]
201RKFWNLIKKNDEKMDubiquitination[2]
246SEPVTMDKVHYCERFubiquitination[1]
493EDSVSRMKPWQSEYGubiquitination[1]
576PTKPYGTKFDRRRPFacetylation[3]
604IQGMLDDKGRVIEDGubiquitination[1]
667KPKENNFKAVLETIRubiquitination[1]
890EEELETEKDFSRYGRubiquitination[1, 4]
1033YLLVKEAKIIAMTCTubiquitination[1]
1045TCTHAALKRHDLVKLacetylation[4]
1051LKRHDLVKLGFKYDNacetylation[3]
1111IKNMAFQKYSNMEQSubiquitination[1, 4, 5, 6, 7]
1256PLIGRPNKVTTVDRFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. 
Sequence Annotation
 MOD_RES 1051 1051 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Reference proteome; RNA-binding; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1485 AA 
Protein Sequence
MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA 60
IRKIMLLEFS QYLENYLWMN YSPEVSSKAY LMSICCMVNE KFRENVPAWE IFKKKPDHFP 120
FFFKHILKAA LAETDGEFSL HEQTVLLLFL DHCFNSLEVD LIRSQVQQLI SLPMWMGLQL 180
ARLELELKKT PKLRKFWNLI KKNDEKMDPE AREQAYQERR FLSQLIQKFI SVLKSVPLSE 240
PVTMDKVHYC ERFIELMIDL EALLPTRRWF NTILDDSHLL VHCYLSNLVR REEDGHLFSQ 300
LLDMLKFYTG FEINDQTGNA LTENEMTTIH YDRITSLQRA AFAHFPELYD FALSNVAEVD 360
TRESLVKFFG PLSSNTLHQV ASYLCLLPTL PKNEDTTFDK EFLLELLVSR HERRISQIQQ 420
LNQMPLYPTE KIIWDENIVP TEYYSGEGCL ALPKLNLQFL TLHDYLLRNF NLFRLESTYE 480
IRQDIEDSVS RMKPWQSEYG GVVFGGWARM AQPIVAFTVV EVAKPNIGEN WPTRVRADVT 540
INLNVRDHIK DEWEGLRKHD VCFLITVRPT KPYGTKFDRR RPFIEQVGLV YVRGCEIQGM 600
LDDKGRVIED GPEPRPNLRG ESRTFRVFLD PNQYQQDMTN TIQNGAEDVY ETFNIIMRRK 660
PKENNFKAVL ETIRNLMNTD CVVPDWLHDI ILGYGDPSSA HYSKMPNQIA TLDFNDTFLS 720
IEHLKASFPG HNVKVTVEDP ALQIPPFRIT FPVRSGKGKK RKDADVEDED TEEAKTLIVE 780
PHVIPNRGPY PYNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI 840
YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETEK DFSRYGRVNY 900
VLARRIELLE EVKRLQKSLG VPGDASYTCE TAGYFFLYQV MSRWEEYISK VKNKGSTLPD 960
VTEVSTFFPF HEYFANAPQP IFKGRSYEED MEIAEGCFRH IKKIFTQLEE FRASELLRSG 1020
LDRSKYLLVK EAKIIAMTCT HAALKRHDLV KLGFKYDNIL MEEAAQILEI ETFIPLLLQN 1080
PQDGFSRLKR WIMIGDHHQL PPVIKNMAFQ KYSNMEQSLF TRFVRVGVPT VDLDAQGRAR 1140
ASLCNLYNWR YKNLGNLPHV QLLPEFSTAN AGLLYDFQLI NVEDFQGVGE SEPNPYFYQN 1200
LGEAEYVVAL FMYMCLLGYP ADKISILTTY NGQKHLIRDI INRRCGNNPL IGRPNKVTTV 1260
DRFQGQQNDY ILLSLVRTRA VGHLRDVRRL VVAMSRARLG LYIFARVSLF QNCFELTPAF 1320
SQLTARPLHL HIIPTEPFPT TRKNGERPSH EVQIIKNMPQ MANFVYNMYM HLIQTTHHYH 1380
QTLLQLPPAM VEEGEEVQNQ ETELETEEEA MTVQADIIPS PTDTSCRQET PAFQTDTTPS 1440
ETGATSTPEA IPALSETTPT VVGAVSAPAE ANTPQDATSA PEETK 1485 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0097157; F:pre-mRNA intronic binding; IEA:InterPro.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB. 
Interpro
 IPR024860; Aquarius.
 IPR026300; CWF11_fam.
 IPR027417; P-loop_NTPase. 
Pfam
  
SMART
  
PROSITE
  
PRINTS