UniProt Accession

 AAKG_YEAST; P12904; D6VU32 

Genbank Protein ID

 M21760; M30470; Z72637; D16506; BK006941 

Genbank Nucleotide ID

 AAA34472.1; AAA35061.1; CAA96823.1; BAA03958.1; DAA07993.1 

Protein Name

 5'-AMP-activated protein kinase subunit gamma 

Protein Synonyms/Alias

 AMPK gamma; AMPK subunit gamma; Regulatory protein CAT3; Sucrose non-fermenting protein 4 

Gene Name

 SNF4 

Gene Synonyms/Alias

 CAT3; SCI1; YGL115W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
2	******MKPTQDSQE	ubiquitination	[1]
172	LTQYRILKFVALNCR	ubiquitination	[1]
276	EGVYTCTKNDKLSTI	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Adenine nucleotides-binding subunit gamma of AMP- activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Gamma non- catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (SNF1) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. 

Sequence Annotation

 DOMAIN 37 97 CBS 1.
 DOMAIN 118 181 CBS 2.
 DOMAIN 194 253 CBS 3.
 DOMAIN 262 322 CBS 4.  

Keyword

 3D-structure; ATP-binding; Carbohydrate metabolism; CBS domain; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 322 AA 

Protein Sequence

Gene Ontology

 GO:0031588; C:AMP-activated protein kinase complex; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005641; C:nuclear envelope lumen; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0043539; F:protein serine/threonine kinase activator activity; IMP:SGD.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
 GO:0007031; P:peroxisome organization; IMP:SGD.
 GO:0045722; P:positive regulation of gluconeogenesis; IMP:SGD.
 GO:0006468; P:protein phosphorylation; IMP:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IGI:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR000644; Cysta_beta_synth_core. 

Pfam

 PF00571; CBS 

SMART

 SM00116; CBS 

PROSITE

 PS51371; CBS 

PRINTS