UniProt Accession

 TRUA_HUMAN; Q9Y606; A8K877; B3KQC1; Q8WYT2; Q9BU44 

Genbank Protein ID

 AF318369; AK074659; AK292242; BC002901; BC009505; BC019320; AF116238 

Genbank Nucleotide ID

 AAL55876.1; BAG51983.1; BAF84931.1; AAH02901.1; AAH09505.2; AAH19320.2; AAD21042.1 

Protein Name

 tRNA pseudouridine synthase A, mitochondrial 

Protein Synonyms/Alias

 tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I 

Gene Name

 PUS1 

Gene Synonyms/Alias

 PP8985 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
110	NVGSSQFKTIEDDLV	ubiquitination	[1, 2]
369	EGKVAAFKEEHIYPT	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (By similarity). 

Sequence Annotation

 ACT_SITE 146 146 Nucleophile (By similarity).
 BINDING 201 201 Substrate (By similarity).
 MOD_RES 415 415 Phosphoserine.
 MOD_RES 420 420 Phosphoserine.
 MOD_RES 426 426 Phosphothreonine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Disease mutation; Isomerase; Mitochondrion; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transit peptide; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 427 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; IEA:Compara.
 GO:0009982; F:pseudouridine synthase activity; IEA:EC.
 GO:0004730; F:pseudouridylate synthase activity; NAS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0008033; P:tRNA processing; NAS:UniProtKB.
 GO:0031119; P:tRNA pseudouridine synthesis; IEA:Compara. 

Interpro

 IPR020103; PsdUridine_synth_cat_dom.
 IPR001406; PsdUridine_synth_TruA.
 IPR020097; PsdUridine_synth_TruA_a/b_dom.
 IPR020095; PsdUridine_synth_TruA_C.
 IPR020094; PsdUridine_synth_TruA_N. 

Pfam

 PF01416; PseudoU_synth_1 

SMART

  

PROSITE

  

PRINTS