UniProt Accession

 CLIC1_MOUSE; Q9Z1Q5 

Genbank Protein ID

 AF109905; BC004658 

Genbank Nucleotide ID

 AAC84155.1; AAH04658.1 

Protein Name

 Chloride intracellular channel protein 1 

Protein Synonyms/Alias

 Nuclear chloride ion channel 27; NCC27 

Gene Name

 Clic1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
119	AKFSAYIKNSNPALN	acetylation	[1, 2, 3, 4]
119	AKFSAYIKNSNPALN	ubiquitination	[5]
131	ALNDNLEKGLLKALK	acetylation	[2, 4]
131	ALNDNLEKGLLKALK	ubiquitination	[5]
135	NLEKGLLKALKVLDN	acetylation	[1, 2]
138	KGLLKALKVLDNYLT	ubiquitination	[5]
166	DEGISQRKFLDGNEL	acetylation	[2]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions (By similarity). 

Sequence Annotation

 DOMAIN 93 233 GST C-terminal.
 REGION 2 90 Required for insertion into the membrane
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 13 13 N6-acetyllysine (By similarity).
 MOD_RES 119 119 N6-acetyllysine (By similarity).
 MOD_RES 131 131 N6-acetyllysine (By similarity).
 MOD_RES 135 135 N6-acetyllysine (By similarity).
 MOD_RES 233 233 Phosphotyrosine.
 DISULFID 24 59 By similarity.  

Keyword

 Acetylation; Cell membrane; Chloride; Chloride channel; Complete proteome; Cytoplasm; Disulfide bond; Ion channel; Ion transport; Membrane; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 241 AA 

Protein Sequence

Gene Ontology

 GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
 GO:0045669; P:positive regulation of osteoblast differentiation; IDA:MGI.
 GO:0051881; P:regulation of mitochondrial membrane potential; IDA:MGI. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR002946; Int_Cl_channel.
 IPR012336; Thioredoxin-like_fold. 

Pfam

  

SMART

  

PROSITE

 PS50405; GST_CTER 

PRINTS

 PR01263; INTCLCHANNEL.