CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016263
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 THO complex subunit 6 homolog 
Protein Synonyms/Alias
 Functional spliceosome-associated protein 35; fSAP35; WD repeat-containing protein 58 
Gene Name
 THOC6 
Gene Synonyms/Alias
 WDR58; PSEC0006 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
107WLWAEMLKKGCKELWubiquitination[1, 2]
201LWDLRTAKEVQTIEVubiquitination[3]
210VQTIEVYKHEECSRPubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. 
Sequence Annotation
 REPEAT 22 61 WD 1.
 REPEAT 74 112 WD 2.
 REPEAT 124 165 WD 3.
 REPEAT 166 205 WD 4.
 REPEAT 215 254 WD 5.
 REPEAT 256 293 WD 6.
 REPEAT 295 339 WD 7.  
Keyword
 Alternative splicing; Complete proteome; mRNA processing; mRNA splicing; mRNA transport; Reference proteome; Repeat; RNA-binding; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 341 AA 
Protein Sequence
MERAVPLAVP LGQTEVFQAL QRLHMTIFSQ SVSPCGKFLA AGNNYGQIAI FSLSSALSSE 60
AKEESKKPVV TFQAHDGPVY SMVSTDRHLL SAGDGEVKAW LWAEMLKKGC KELWRRQPPY 120
RTSLEVPEIN ALLLVPKENS LILAGGDCQL HTMDLETGTF TRVLRGHTDY IHCLALRERS 180
PEVLSGGEDG AVRLWDLRTA KEVQTIEVYK HEECSRPHNG RWIGCLATDS DWMVCGGGPA 240
LTLWHLRSST PTTIFPIRAP QKHVTFYQDL ILSAGQGRCV NQWQLSGELK AQVPGSSPGL 300
LSLSLNQQPA APECKVLTAA GNSCRVDVFT NLGYRAFSLS F 341 
Gene Ontology
 GO:0000445; C:THO complex part of transcription export complex; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0046784; P:intronless viral mRNA export from host nucleus; IDA:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS