CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020447
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 518B 
Protein Synonyms/Alias
  
Gene Name
 ZNF518B 
Gene Synonyms/Alias
 KIAA1729 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
68AKCKSVHKISLQDLQubiquitination[1, 2, 3]
76ISLQDLQKGTGKDGMubiquitination[1, 3]
151TKDPLQYKKHTLQHEubiquitination[2]
152KDPLQYKKHTLQHEEubiquitination[2]
237PKRTGTSKQNPELLKubiquitination[2]
244KQNPELLKASNPRTTubiquitination[2]
282IMLLPEPKEYQKDVVmethylation[4]
286PEPKEYQKDVVCIPNubiquitination[2, 5]
396KGSNEQEKVLSAEKTubiquitination[2]
404VLSAEKTKSLTVDGNubiquitination[2, 5]
414TVDGNVGKLVGIDSFubiquitination[2]
430PSVQKQLKNVKWVRSubiquitination[2]
433QKQLKNVKWVRSYDFubiquitination[2]
452SSVHNNGKSFINSETubiquitination[2]
465ETIEDFQKKNNLYPHubiquitination[2, 6, 7]
466TIEDFQKKNNLYPHRubiquitination[2, 5]
482AFPSVALKGHSLASVubiquitination[2, 5]
491HSLASVFKNSVLRSLubiquitination[2]
508ASNPFPYKAAVCFAEubiquitination[2, 7]
558NDLESTSKVNIPVKVubiquitination[2, 5]
564SKVNIPVKVVSSNRKubiquitination[2, 5, 6]
614RSQQPGDKPLELKNSubiquitination[2, 5]
619GDKPLELKNSERTNNubiquitination[2, 5]
660NSSTSKIKSIELLRRubiquitination[2, 5]
678QLIESCGKPSSLASNubiquitination[2, 5]
726HSTGTLQKPPNDGGIubiquitination[2]
809CSERQLIKPVPFCPVubiquitination[2]
860VCSTIHRKTGLLYGQubiquitination[2]
875QGSSELNKQGRLLSRubiquitination[2, 5, 6]
912AEPSRCLKDPSIFQVubiquitination[2, 5]
929QLRLIAAKPDQLIKCubiquitination[2, 5]
935AKPDQLIKCPRRNQPubiquitination[2, 5]
1004AEEASQIKRQMMLKMubiquitination[2, 5, 6, 7]
1018MKLKKVHKNNYQVVDubiquitination[2]
1072DWDVLSSKGK*****ubiquitination[2, 5]
1074DVLSSKGK*******ubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 ZN_FING 162 184 C2H2-type 1.
 ZN_FING 190 213 C2H2-type 2.
 ZN_FING 1036 1058 C2H2-type 3.  
Keyword
 Complete proteome; DNA-binding; Metal-binding; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1074 AA 
Protein Sequence
MKDIGQQLYT THLNGGHNSL TMSPKQPDAN GAPRPNRQEA QTLLYQGSEA EAAMMTIATC 60
AKCKSVHKIS LQDLQKGTGK DGMYVCFQCS LGAAPPNFHF VSNNSSATHV GNKTENFSSS 120
VNSKFKVRNF KPGKYYCDKC RFSTKDPLQY KKHTLQHEEI KFICSHCSYI SYTKGEFQRH 180
LVKHTGIFPY QCEYCDYGAI RNDYIVKHTK RVHERAGAKR PVKAVAKLEP KRTGTSKQNP 240
ELLKASNPRT TFQNKWSDQL SGFSLHANKD KMHNIMLLPE PKEYQKDVVC IPNKMTLSEP 300
NEVNLFENKN VEVEVLSPAK EPVQPGMPLT VVAPAELVVP ANCLAQLIDV KVVNGTQQLV 360
LKLFPLEENN CLEAGRDNGG NSERMVKEKG SNEQEKVLSA EKTKSLTVDG NVGKLVGIDS 420
FQPSVQKQLK NVKWVRSYDF IMPNSSVHNN GKSFINSETI EDFQKKNNLY PHRTAFPSVA 480
LKGHSLASVF KNSVLRSLGA ASNPFPYKAA VCFAESGRNL HSSSQQLLPF AASPATCSFS 540
GEKGLLPVSE NDLESTSKVN IPVKVVSSNR KQEDNQTEEH KAVSTVGQIS SQHKSEYLHI 600
NITGEDRSQQ PGDKPLELKN SERTNNTNDG PVISSVFSLS SGSENVPEGI KWNSSTSKIK 660
SIELLRRKIA QLIESCGKPS SLASNSAHRR SVGQASKGTS KATSEGIQEI NVSLTGLGHS 720
TGTLQKPPND GGITGNRQLT HQQIYPHFAD GSNRKTKSRV ARKAHVATPV LIPKGAVLRV 780
LNSSENAHII EATCEAPVSI PCSERQLIKP VPFCPVRQAD SDLQPLRSER GPIDMSPNIE 840
TPLRPKLRKE SAVCSTIHRK TGLLYGQQGS SELNKQGRLL SRSLSISRNK TKQVHLSRKK 900
NKIQAEPSRC LKDPSIFQVA RQLRLIAAKP DQLIKCPRRN QPVIVLNHPD VDSPEVTNVM 960
KVINKYKGNV LKVVLSERTR CQLGIRRHHV RLTYQNAEEA SQIKRQMMLK MKLKKVHKNN 1020
YQVVDSLPDD SSQCVFKCWF CGRLYEDQEE WMSHGQRHLI EATRDWDVLS SKGK 1074 
Gene Ontology
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0000981; F:sequence-specific DNA binding RNA polymerase II transcription factor activity; IBA:RefGenome.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS