Tag | Content |
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CPLM ID | CPLM-008497 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 10 |
Protein Synonyms/Alias | Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10 |
Gene Name | Usp10 |
Gene Synonyms/Alias | Kiaa0190; Ode-1; Uchrp |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
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403 | ETVTLIHKPVSLQPR | ubiquitination | [1] | 446 | KFIPLYSKVQRPCTS | ubiquitination | [1] | 672 | SRRVTLEKLPPVLVL | ubiquitination | [1] | 697 | GGCQKLVKNIDYPVD | ubiquitination | [1] |
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Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34- containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling (By similarity). |
Sequence Annotation | REGION 2 99 Interaction with p53/TP53 (By ACT_SITE 418 418 Nucleophile (By similarity). ACT_SITE 743 743 Proton acceptor (By similarity). MOD_RES 2 2 N-acetylalanine (By similarity). MOD_RES 24 24 Phosphothreonine (By similarity). MOD_RES 205 205 Phosphothreonine. MOD_RES 223 223 Phosphoserine (By similarity). MOD_RES 330 330 Phosphoserine; by ATM (By similarity). MOD_RES 359 359 Phosphoserine (By similarity). MOD_RES 364 364 Phosphoserine (By similarity). MOD_RES 570 570 Phosphoserine. |
Keyword | Acetylation; Alternative splicing; Autophagy; Complete proteome; Cytoplasm; DNA damage; DNA repair; Endosome; Hydrolase; Nucleus; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 792 AA |
Protein Sequence | MALHNPQYIF GDFSPDEFNQ FFVTPRSSVE LPPYSGTLCS IQAEDELPDA GQEHQRIEFG 60 VDEVIEPSEG LPPTPSYSIS STLNPQAPEF ILGCTTSKKI PEAVEKDETY SSIDQYPASA 120 LALESNSNAE AETLENDSGA GGLGQRERKK KKKRPPGYYS YLKDGGEDSA SPATLVNGHA 180 TSVGTSGEAV EDAEFMDVLP PVMPRTCDSP QNPVDFISGP VPDSPFPRTL GGDARTAGLC 240 EGCHEADFEQ PCLPADSLLR TAGTQPYVGT DTTENFAVAN GKILESPGED TAANGAELHT 300 DEGADLDPAK PESQSPPAES ALSASGAIPI SQPAKSWASL FHDSKPSASS PMAYVETKCS 360 PPVPSPLASE KQMEVKEGLV PVSEDPVAIK IAELLETVTL IHKPVSLQPR GLINKGNWCY 420 INATLQALVA CPPMYHLMKF IPLYSKVQRP CTSTPMIDSF VRLMNEFTNM PVPPKPRQAL 480 GDKIVRDIRP GAAFEPTYIY RLLTVIKSSL SEKGRQEDAE EYLGFILNGL HEEMLSLKKL 540 LSPTHEKHSV SNGPRSDLIE DEELEDTGKG SEDEWEQVGP KNKTSITRQA DFVQTPITGI 600 FGGHIRSVVY QQSSKESATL QLFFTLQLDI QSDKIRTVQD ALESLVARES VQGYTTKTKQ 660 EVEVSRRVTL EKLPPVLVLH LKRFVYEKTG GCQKLVKNID YPVDLEISRE LLSPGIKNKN 720 FKCQRTYRLF AVVYHHGNSA TGGHYTTDVF QIGLNGWLRI DDQTVKVINQ YQVVKPPADR 780 TAYLLYYRRV DLL 793 |
Gene Ontology | GO:0005769; C:early endosome; ISS:UniProtKB. GO:0045111; C:intermediate filament cytoskeleton; IEA:Compara. GO:0005634; C:nucleus; ISS:UniProtKB. GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB. GO:0044325; F:ion channel binding; ISS:UniProtKB. GO:0002039; F:p53 binding; ISS:UniProtKB. GO:0004221; F:ubiquitin thiolesterase activity; ISS:UniProtKB. GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB. GO:0006914; P:autophagy; IEA:UniProtKB-KW. GO:0030330; P:DNA damage response, signal transduction by p53 class mediator; ISS:UniProtKB. GO:0006281; P:DNA repair; IEA:UniProtKB-KW. GO:0016579; P:protein deubiquitination; ISS:UniProtKB. GO:0010506; P:regulation of autophagy; ISS:UniProtKB. GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. |
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