UniProt Accession

 GLSA1_ECOLI; P77454; Q2MBU2 

Genbank Protein ID

 U82664; U00096; AP009048 

Genbank Nucleotide ID

 AAB40239.1; AAC73587.1; BAE76264.1 

Protein Name

 Glutaminase 1 

Protein Synonyms/Alias

  

Gene Name

 glsA1 

Gene Synonyms/Alias

 ybaS; b0485; JW0474 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
294	GNSVRGQKMVASVAK	acetylation	[1, 2]
301	KMVASVAKQLGYNVF	acetylation	[2, 3]
309	QLGYNVFKG******	acetylation	[3]

Reference

 [1] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

  

Sequence Annotation

 BINDING 66 66 Substrate (By similarity).
 BINDING 117 117 Substrate (By similarity).
 BINDING 161 161 Substrate (By similarity).
 BINDING 168 168 Substrate (By similarity).
 BINDING 192 192 Substrate (By similarity).
 BINDING 244 244 Substrate (By similarity).
 BINDING 262 262 Substrate; via amide nitrogen (By
 MOD_RES 294 294 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Hydrolase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 310 AA 

Protein Sequence

Gene Ontology

 GO:0004359; F:glutaminase activity; IDA:EcoCyc.
 GO:0006541; P:glutamine metabolic process; IEA:InterPro.
 GO:0045926; P:negative regulation of growth; IMP:EcoliWiki.
 GO:0010447; P:response to acidity; IEP:EcoCyc. 

Interpro

 IPR012338; Beta-lactam/transpept-like.
 IPR015868; Glutaminase. 

Pfam

 PF04960; Glutaminase 

SMART

  

PROSITE

  

PRINTS