UniProt Accession

 TOP2A_MOUSE; Q01320; E9PX08 

Genbank Protein ID

 D12513; AL591067; U01915 

Genbank Nucleotide ID

 BAA02076.1; AAC52135.1 

Protein Name

 DNA topoisomerase 2-alpha 

Protein Synonyms/Alias

 DNA topoisomerase II, alpha isozyme 

Gene Name

 Top2a 

Gene Synonyms/Alias

 Top-2; Top2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
130	GIPVVEHKVEKIYVP	acetylation	[1]
479	LGVVGRDKYGVFPLR	acetylation	[1, 2]
528	YEDEDSLKTLRYGKI	acetylation	[1]
1033	LKYYGLRKEWLLGML	acetylation	[1]
1311	VDVPPRQKEQRSAAA	acetylation	[3]
1319	EQRSAAAKAKFTVDL	acetylation	[3]
1418	GVKKTATKSQSSVST	acetylation	[2]
1438	RAAPKGTKSDSALSA	acetylation	[2]
1450	LSARVSEKPAPAKAK	acetylation	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. 

Sequence Annotation

 DOMAIN 454 571 Toprim.
 NP_BIND 147 149 ATP (By similarity).
 NP_BIND 160 167 ATP (By similarity).
 NP_BIND 375 377 ATP (By similarity).
 REGION 341 343 Interaction with DNA (By similarity).
 REGION 989 998 Interaction with DNA (By similarity).
 ACT_SITE 804 804 O-(5'-phospho-DNA)-tyrosine intermediate
 METAL 460 460 Magnesium 1; catalytic (By similarity).
 METAL 540 540 Magnesium 1; catalytic (By similarity).
 METAL 540 540 Magnesium 2 (By similarity).
 METAL 542 542 Magnesium 2 (By similarity).
 BINDING 90 90 ATP (By similarity).
 BINDING 119 119 ATP (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 4 4 Phosphoserine (By similarity).
 MOD_RES 28 28 Phosphoserine.
 MOD_RES 281 281 Phosphothreonine (By similarity).
 MOD_RES 1105 1105 Phosphoserine; by CK1 (By similarity).
 MOD_RES 1211 1211 Phosphoserine (By similarity).
 MOD_RES 1291 1291 Phosphoserine (By similarity).
 MOD_RES 1293 1293 Phosphoserine (By similarity).
 MOD_RES 1295 1295 Phosphoserine (By similarity).
 MOD_RES 1298 1298 Phosphoserine (By similarity).
 MOD_RES 1328 1328 Phosphoserine (By similarity).
 MOD_RES 1333 1333 Phosphoserine.
 MOD_RES 1367 1367 Phosphothreonine.
 MOD_RES 1370 1370 Phosphoserine.
 MOD_RES 1371 1371 Phosphoserine.
 MOD_RES 1373 1373 Phosphoserine.
 MOD_RES 1379 1379 Phosphoserine.
 MOD_RES 1384 1384 Phosphoserine (By similarity).
 MOD_RES 1465 1465 Phosphoserine.
 MOD_RES 1466 1466 Phosphoserine.
 MOD_RES 1467 1467 Phosphoserine.
 MOD_RES 1469 1469 Phosphoserine.
 MOD_RES 1470 1470 Phosphoserine.
 MOD_RES 1472 1472 Phosphoserine.
 MOD_RES 1521 1521 Phosphoserine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; DNA-binding; Isomerase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Topoisomerase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1528 AA 

Protein Sequence

Gene Ontology

 GO:0005814; C:centriole; IEA:Compara.
 GO:0009330; C:DNA topoisomerase complex (ATP-hydrolyzing); IEA:Compara.
 GO:0005730; C:nucleolus; IDA:MGI.
 GO:0005654; C:nucleoplasm; IEA:Compara.
 GO:0000795; C:synaptonemal complex; IBA:RefGenome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0008301; F:DNA binding, bending; ISS:UniProtKB.
 GO:0003918; F:DNA topoisomerase type II (ATP-hydrolyzing) activity; IDA:MGI.
 GO:0008144; F:drug binding; IEA:Compara.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0030263; P:apoptotic chromosome condensation; IEA:Compara.
 GO:0030261; P:chromosome condensation; IMP:MGI.
 GO:0006266; P:DNA ligation; IEA:Compara.
 GO:0006265; P:DNA topological change; IDA:MGI.
 GO:0006261; P:DNA-dependent DNA replication; IBA:RefGenome.
 GO:0040016; P:embryonic cleavage; IMP:MGI.
 GO:0044774; P:mitotic DNA integrity checkpoint; IBA:RefGenome.
 GO:0006312; P:mitotic recombination; IBA:RefGenome.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0045870; P:positive regulation of retroviral genome replication; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:MGI.
 GO:0000712; P:resolution of meiotic recombination intermediates; IBA:RefGenome.
 GO:0006974; P:response to DNA damage stimulus; IEA:Compara.
 GO:0000819; P:sister chromatid segregation; IBA:RefGenome. 

Interpro

 IPR024946; Arg_repress_C-like.
 IPR012542; DTHCT.
 IPR003594; HATPase_ATP-bd.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
 IPR001241; Topo_IIA.
 IPR002205; Topo_IIA_A/C.
 IPR013758; Topo_IIA_A/C_ab.
 IPR013757; Topo_IIA_A_a.
 IPR013506; Topo_IIA_bsu_dom2.
 IPR013759; Topo_IIA_cen_dom.
 IPR013760; Topo_IIA_like_dom.
 IPR018522; TopoIIA_CS.
 IPR006171; Toprim_domain. 

Pfam

 PF00204; DNA_gyraseB
 PF00521; DNA_topoisoIV
 PF08070; DTHCT
 PF02518; HATPase_c
 PF01751; Toprim 

SMART

 SM00387; HATPase_c
 SM00433; TOP2c
 SM00434; TOP4c 

PROSITE

 PS00177; TOPOISOMERASE_II
 PS50880; TOPRIM 

PRINTS

 PR00418; TPI2FAMILY.