| Tag | Content |
|---|
| CPLM ID | CPLM-011444 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Auxilin-like clathrin uncoating factor SWA2 |
Protein Synonyms/Alias | Bud site selection protein 24; DnaJ-related protein SWA2; J protein SWA2; Synthetic lethal with ARF1 protein 2 |
Gene Name | SWA2 |
Gene Synonyms/Alias | AUX1; BUD24; YDR320C; D9798.10 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 200 | ESGIKMEKSGLSNIV | ubiquitination | [1] | | 252 | NYSKTHDKVEDYDLP | ubiquitination | [1] | | 386 | AKGTSLFKNGDYINS | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Cofactor for the uncoating of clathrin-coated vesicles (CCVs) by Hsp70-type chaperones (SSA1/2/3 and SSB1/2). Coat disassembly is important for fusion of vesicles with target membranes and for recycling components of clathrin coats to the cytoplasm for further rounds of vesicle formation. Binds to assembled clathrin and recruits the ATP-activated chaperone to CCVs. Stimulates the ATPase activity of the clathrin-associated Hsp70-type chaperone SSA1, which then disrupts clathrin-clathrin interactions, leading to release of the clathrin coat. In addition, prevents unproductive clathrin assembly in the cell. Also required for cortical endoplasmic reticulum inheritance. |
Sequence Annotation | DOMAIN 140 180 UBA.
REPEAT 374 407 TPR 1.
REPEAT 412 445 TPR 2.
REPEAT 467 500 TPR 3.
DOMAIN 603 668 J.
REGION 1 100 CB1.
REGION 238 302 CB2.
REGION 303 362 CB3.
MOD_RES 52 52 Phosphoserine.
MOD_RES 64 64 Phosphoserine.
MOD_RES 264 264 Phosphoserine.
MOD_RES 308 308 Phosphoserine.
MOD_RES 312 312 Phosphoserine. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Endoplasmic reticulum; Membrane; Phosphoprotein; Reference proteome; Repeat; TPR repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 668 AA |
Protein Sequence | MSDPFAHLLT SLKNKDSASA SKETTPQSSN SPSITGSAVA DVARTDKSPN DSLHSISAPP 60
LIPSPKVDFS APPLVPTNST TKSNTANNTP PSALANTDDD FNQLFGMGTV TTTDTIQKPD 120
EDYYGSKEDH LYNGDDALVD EVKDMEIARL MSLGLSIEEA TEFYENDVTY ERYLEILKSK 180
QKERNDLAIR KKESGIKMEK SGLSNIVGTD SNNLFSMATD FFNKGKKLVD QWTSFPPEAN 240
DRLNNYSKTH DKVEDYDLPQ VNDSPNRILF EDNEVVENLP PADNPDQDLL TDFETKIDIT 300
KRTAPDVSHS SSPTSGILIE ENSRRNEPLI EDSLLDFSEG NLTNSKSNED STLFNENSNT 360
DSTIPISDIE LSGYNEFKAK GTSLFKNGDY INSLQEYEKS LNTLPLNHPL RIIALSNIIA 420
SQLKIGEYSK SIENSSMALE LFPSSKAKWK NKISNSDPER SFNDIWPKIM IRRAESFEHL 480
ESFKKALETY QELIKKNFFD DKIMQGKRRC QDFINPPPVK KSMPVKKKTT TTSPATKKQN 540
LTASSSNSPI SVDSTSEIKK RELENAKLAL YDKVFEKISS WKDGKDDDIR HLLANLSSLL 600
TWCNWKDVSM QDLVMPKRVK ITYMKAVAKT HPDKIPESLS LENKMIAENI FSTLSIAWDK 660
FKLQNDIN 668 |
Gene Ontology | GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD. GO:0030276; F:clathrin binding; IDA:SGD. GO:0043130; F:ubiquitin binding; IDA:SGD. GO:0072318; P:clathrin coat disassembly; IMP:SGD. GO:0048309; P:endoplasmic reticulum inheritance; IMP:SGD. GO:0032781; P:positive regulation of ATPase activity; IDA:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |