UniProt Accession

 B4DU17_HUMAN; B4DU17 

Genbank Protein ID

 AL357153; AK300460 

Genbank Nucleotide ID

 BAG62179.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 6 

Protein Synonyms/Alias

 Mediator complex subunit 6 

Gene Name

 MED6 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
51	TCNNEVVKMQRLTLE	ubiquitination	[1, 2, 3]
150	YCRYHPSKGYWWHFK	ubiquitination	[2]
172	VRPKAKRKEEPSSIF	ubiquitination	[1, 2, 4]
203	PPKFVQLKPGEKPVP	ubiquitination	[2, 3, 4]
207	VQLKPGEKPVPVDQT	ubiquitination	[2, 5, 6]
216	VPVDQTKKEAEPIPE	ubiquitination	[4]
226	EPIPETVKPEEKETT	ubiquitination	[4]
234	PEEKETTKNVQQTVS	ubiquitination	[2, 3, 4]
243	VQQTVSAKGPPEKRM	acetylation	[3, 7, 8]
243	VQQTVSAKGPPEKRM	ubiquitination	[4]
248	SAKGPPEKRMRLQ**	acetylation	[3, 7, 8]

Reference

 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). 

Sequence Annotation

  

Keyword

 Activator; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 253 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IEA:InterPro.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro. 

Interpro

 IPR007018; Mediator_Med6.
 IPR016820; Mediator_Med6_met/pln. 

Pfam

 PF04934; Med6 

SMART

  

PROSITE

  

PRINTS