UniProt Accession

 NDKA_DROME; P08879; Q9V9U5 

Genbank Protein ID

 X13107; AE014297; AY113576 

Genbank Nucleotide ID

 CAA31500.1; AAF57188.3; AAM29581.1 

Protein Name

 Nucleoside diphosphate kinase 

Protein Synonyms/Alias

 NDK; NDP kinase; Abnormal wing disks protein; Killer of prune protein 

Gene Name

 awd 

Gene Synonyms/Alias

 K-pn; CG2210 

Created Date

 July 27, 2013 

Organism

 Drosophila melanogaster (Fruit fly) 

NCBI Taxa ID

 7227 

Lysine Modification

Position	Peptide	Type	References
13	ERTFIMVKPDGVQRG	acetylation	[1]
24	VQRGLVGKIIERFEQ	acetylation	[1]
35	RFEQKGFKLVALKFT	acetylation	[1]
46	LKFTWASKELLEKHY	acetylation	[1]
51	ASKELLEKHYADLSA	acetylation	[1]

Reference

 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 

Functional Description

 Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. 

Sequence Annotation

 ACT_SITE 119 119 Pros-phosphohistidine intermediate.
 BINDING 13 13 ATP.
 BINDING 61 61 ATP.
 BINDING 89 89 ATP.
 BINDING 95 95 ATP.
 BINDING 106 106 ATP.
 BINDING 116 116 ATP.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 126 126 Phosphoserine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Microtubule; Nucleotide metabolism; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 153 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005875; C:microtubule associated complex; IDA:FlyBase.
 GO:0005880; C:nuclear microtubule; IMP:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IDA:FlyBase.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0008017; F:microtubule binding; IDA:FlyBase.
 GO:0004550; F:nucleoside diphosphate kinase activity; IDA:UniProtKB.
 GO:0034332; P:adherens junction organization; IMP:FlyBase.
 GO:0006241; P:CTP biosynthetic process; TAS:UniProtKB.
 GO:0007427; P:epithelial cell migration, open tracheal system; IMP:FlyBase.
 GO:0016334; P:establishment or maintenance of polarity of follicular epithelium; IMP:FlyBase.
 GO:0006183; P:GTP biosynthetic process; TAS:UniProtKB.
 GO:0007017; P:microtubule-based process; IMP:FlyBase.
 GO:0007067; P:mitosis; IMP:FlyBase.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:FlyBase.
 GO:0046777; P:protein autophosphorylation; IDA:FlyBase.
 GO:0035152; P:regulation of tube architecture, open tracheal system; IMP:FlyBase.
 GO:0006228; P:UTP biosynthetic process; TAS:UniProtKB. 

Interpro

 IPR001564; Nucleoside_diP_kinase.
 IPR023005; Nucleoside_diP_kinase_AS. 

Pfam

 PF00334; NDK 

SMART

 SM00562; NDK 

PROSITE

 PS00469; NDP_KINASES 

PRINTS

 PR01243; NUCDPKINASE.