UniProt Accession

 NPL4_MOUSE; P60670; B1ATY4; B1ATY5 

Genbank Protein ID

 AK129375; AL669855; AL669855; BC065156 

Genbank Nucleotide ID

 BAC98185.1; CAM27040.1; CAM27041.1; AAH65156.1 

Protein Name

 Nuclear protein localization protein 4 homolog 

Protein Synonyms/Alias

 Protein NPL4 

Gene Name

 Nploc4 

Gene Synonyms/Alias

 Kiaa1499; Npl4 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
62	EITASSSKSLHLLKI	ubiquitination	[1]
68	SKSLHLLKIKHGDLL	ubiquitination	[1]
116	EIDQYLSKQDGKIYR	ubiquitination	[1]
179	TGGADKGKFVALENI	acetylation	[2, 3]
243	RFLDFWRKTGNQHFG	ubiquitination	[1]
260	YGRYTEHKDIPLGIR	ubiquitination	[1]
302	VVDEIAAKLGLRKVG	ubiquitination	[1]
307	AAKLGLRKVGWIFTD	ubiquitination	[1]
544	EELAQTWKKSEQWAT	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). 

Sequence Annotation

 ZN_FING 580 608 RanBP2-type.
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 3D-structure; Acetylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; Endoplasmic reticulum; Metal-binding; Nucleus; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 608 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; ISS:HGNC.
 GO:0042175; C:nuclear outer membrane-endoplasmic reticulum membrane network; ISS:HGNC.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006944; P:cellular membrane fusion; ISS:HGNC.
 GO:0030433; P:ER-associated protein catabolic process; ISS:HGNC.
 GO:0007030; P:Golgi organization; ISS:HGNC. 

Interpro

 IPR007717; NPL4.
 IPR024682; Npl4_Ub-like_dom.
 IPR007716; NPL4_Zn-bd_put.
 IPR016563; PolyUb_recognition_cplx_Npl4.
 IPR001876; Znf_RanBP2. 

Pfam

 PF05021; NPL4
 PF11543; UN_NPL4
 PF05020; zf-NPL4 

SMART

 SM00547; ZnF_RBZ 

PROSITE

 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2 

PRINTS