CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001603
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 dTDP-D-glucose 4,6-dehydratase 
Protein Synonyms/Alias
  
Gene Name
 TGDS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18GLPGGFAKRVLVTGGubiquitination[1]
67NLETISNKQNYKFIQubiquitination[1, 2, 3]
71ISNKQNYKFIQGDICubiquitination[1]
160EFDESSPKQPTNPYAubiquitination[2]
170TNPYASSKAAAECFVubiquitination[4]
185QSYWEQYKFPVVITRubiquitination[1, 4]
205GPHQYPEKVIPKFISubiquitination[1, 3]
209YPEKVIPKFISLLQHubiquitination[1]
219SLLQHNRKCCIHGSGubiquitination[1]
250LTVLKKGKPGEIYNIubiquitination[2]
343HNWKNVEKALEPFPVubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
  
Sequence Annotation
 ACT_SITE 143 143 Proton donor (By similarity).
 ACT_SITE 144 144 Proton acceptor (By similarity).
 ACT_SITE 166 166 Proton acceptor (By similarity).
 BINDING 142 142 Substrate (By similarity).  
Keyword
 Complete proteome; Lyase; NAD; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 350 AA 
Protein Sequence
MSAACWEEPW GLPGGFAKRV LVTGGAGFIA SHMIVSLVED YPNYMIINLD KLDYCASLKN 60
LETISNKQNY KFIQGDICDS HFVKLLFETE KIDIVLHFAA QTHVDLSFVR AFEFTYVNVY 120
GTHVLVSAAH EARVEKFIYV STDEVYGGSL DKEFDESSPK QPTNPYASSK AAAECFVQSY 180
WEQYKFPVVI TRSSNVYGPH QYPEKVIPKF ISLLQHNRKC CIHGSGLQTR NFLYATDVVE 240
AFLTVLKKGK PGEIYNIGTN FEMSVVQLAK ELIQLIKETN SESEMENWVD YVNDRPTNDM 300
RYPMKSEKIH GLGWRPKVPW KEGIKKTIEW YRENFHNWKN VEKALEPFPV 350 
Gene Ontology
 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:EC.
 GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro. 
Interpro
 IPR005888; dTDP_Gluc_deHydtase.
 IPR001509; Epimerase_deHydtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF01370; Epimerase 
SMART
  
PROSITE
  
PRINTS