CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014272
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myocyte-specific enhancer factor 2A 
Protein Synonyms/Alias
  
Gene Name
 Mef2a 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
247TGANSLGKVMPTKSPacetylation[1, 2, 3]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation (By similarity). 
Sequence Annotation
 DOMAIN 3 57 MADS-box.
 DNA_BIND 58 86 Mef2-type (Potential).
 REGION 264 281 Required for interaction with MAPKs (By
 REGION 287 294 Beta domain (By similarity).
 MOD_RES 59 59 Phosphoserine; by CK2 (By similarity).
 MOD_RES 98 98 Phosphoserine.
 MOD_RES 233 233 Phosphoserine (By similarity).
 MOD_RES 247 247 N6-acetyllysine (By similarity).
 MOD_RES 253 253 Phosphoserine (By similarity).
 MOD_RES 310 310 Phosphothreonine; by MAPK7; alternate (By
 MOD_RES 310 310 Phosphothreonine; by NLK; alternate.
 MOD_RES 317 317 Phosphothreonine; by MAPK7 (By
 MOD_RES 353 353 Phosphoserine; by MAPK7 (By similarity).
 MOD_RES 401 401 N6-acetyllysine; alternate (By
 MOD_RES 406 406 Phosphoserine (By similarity).
 MOD_RES 444 444 Phosphoserine (By similarity).
 CROSSLNK 401 401 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Activator; Alternative splicing; Apoptosis; Complete proteome; Developmental protein; Differentiation; DNA-binding; Isopeptide bond; Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 498 AA 
Protein Sequence
MGRKKIQITR IMDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS SNKLFQYAST 60
DMDKVLLKYT EYNEPHESRT NSDIVETLRK KGLNGCESPD ADDYFEHSPL SEDRFSKLNE 120
DSDFIFKRGP PGLPPQNFSM SVTVPVTSPN ALSYTNPGSS LVSPSLAASS TLADSSMLSP 180
PPATLHRNVS PGAPQRPPST GSASGMLSTT DLTVPNGAGN SPVGNGFVNS RASPNLIGNT 240
GANSLGKVMP TKSPPPPGGG SLGMNSRKPD LRVVIPPSSK GMMPPLSEEE ELELNAQRIS 300
SSQATQPLAT PVVSVTTPSL PPQGLVYSAM PTAYNTDYSL TSADLSALQG FTSPGMLSLG 360
QASAWQQHHL GQAALSSLVA GGQLSQGSNL SINTNQNINI KSEPISPPRD RMTPSGFQQQ 420
QQQPQQQPPP QPPQPQPRQE MGRSPVDSLS SSSSSYDGSD REDPRGDFHS PIVLGRPPNT 480
EDRESPSVKR MRMDTWVT 498 
Gene Ontology
 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0035035; F:histone acetyltransferase binding; ISS:UniProtKB.
 GO:0042826; F:histone deacetylase binding; ISS:UniProtKB.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:MGI.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; ISS:UniProtKB.
 GO:0001105; F:RNA polymerase II transcription coactivator activity; IEA:Compara.
 GO:0046332; F:SMAD binding; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0061337; P:cardiac conduction; IMP:UniProtKB.
 GO:0071277; P:cellular response to calcium ion; ISS:UniProtKB.
 GO:0070375; P:ERK5 cascade; ISS:UniProtKB.
 GO:0000002; P:mitochondrial genome maintenance; IMP:UniProtKB.
 GO:0048311; P:mitochondrion distribution; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0055005; P:ventricular cardiac myofibril development; IMP:UniProtKB. 
Interpro
 IPR022102; HJURP_C.
 IPR002100; TF_MADSbox. 
Pfam
 PF12347; HJURP_C
 PF00319; SRF-TF 
SMART
 SM00432; MADS 
PROSITE
 PS00350; MADS_BOX_1
 PS50066; MADS_BOX_2 
PRINTS
 PR00404; MADSDOMAIN.