CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020081
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 45 kDa calcium-binding protein 
Protein Synonyms/Alias
 Cab45; Stromal cell-derived factor 4; SDF-4 
Gene Name
 SDF4 
Gene Synonyms/Alias
 CAB45; PSEC0034 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
85HQEVFLGKDLGGFDEubiquitination[1, 2, 3, 4]
165SWDEYKVKFLASKGHubiquitination[3]
188IRLNEELKVDEETQEubiquitination[2]
201QEVLENLKDRWYQADubiquitination[2]
239GMLRFMVKEIVRDLDubiquitination[3]
350SEFFTGSKLVDYARSubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment (By similarity). 
Sequence Annotation
 DOMAIN 98 133 EF-hand 1.
 DOMAIN 137 172 EF-hand 2.
 DOMAIN 197 232 EF-hand 3.
 DOMAIN 233 268 EF-hand 4.
 DOMAIN 278 313 EF-hand 5.
 DOMAIN 314 349 EF-hand 6.
 REGION 309 362 Necessary for intracellular retention in
 CARBOHYD 40 40 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Calcium; Cell projection; Complete proteome; Cytoplasm; Exocytosis; Glycoprotein; Golgi apparatus; Metal-binding; Polymorphism; Reference proteome; Repeat; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 362 AA 
Protein Sequence
MVWPWVAMAS RWGPLIGLAP CCLWLLGAVL LMDASARPAN HSSTRERVAN REENEILPPD 60
HLNGVKLEMD GHLNRGFHQE VFLGKDLGGF DEDAEPRRSR RKLMVIFSKV DVNTDRKISA 120
KEMQRWIMEK TAEHFQEAME ESKTHFRAVD PDGDGHVSWD EYKVKFLASK GHSEKEVADA 180
IRLNEELKVD EETQEVLENL KDRWYQADSP PADLLLTEEE FLSFLHPEHS RGMLRFMVKE 240
IVRDLDQDGD KQLSVPEFIS LPVGTVENQQ GQDIDDNWVK DRKKEFEELI DSNHDGIVTA 300
EELESYMDPM NEYNALNEAK QMIAVADENQ NHHLEPEEVL KYSEFFTGSK LVDYARSVHE 360
EF 362 
Gene Ontology
 GO:0032059; C:bleb; IEA:UniProtKB-SubCell.
 GO:0005796; C:Golgi lumen; ISS:BHF-UCL.
 GO:0005770; C:late endosome; ISS:BHF-UCL.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005509; F:calcium ion binding; IDA:BHF-UCL.
 GO:0042802; F:identical protein binding; ISS:BHF-UCL.
 GO:0021549; P:cerebellum development; ISS:BHF-UCL.
 GO:0045444; P:fat cell differentiation; ISS:BHF-UCL.
 GO:0045471; P:response to ethanol; ISS:BHF-UCL.
 GO:0009650; P:UV protection; ISS:BHF-UCL.
 GO:0070625; P:zymogen granule exocytosis; ISS:BHF-UCL. 
Interpro
 IPR027240; CAB45.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 
Pfam
 PF13202; EF_hand_3
 PF13499; EF_hand_5 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS