CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016469
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Melanoma inhibitory activity protein 3 
Protein Synonyms/Alias
 Transport and Golgi organization protein 1 
Gene Name
 Mia3 
Gene Synonyms/Alias
 Kiaa0268; Tango1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1267KLSSYEQKIKESKKYubiquitination[1]
1309TNVSLGDKAKSLRLQubiquitination[1]
1504DLKLLQLKLRASMSTubiquitination[1]
1671QDEPVIVKPMPGRPNubiquitination[1]
1779AKAVDEGKVNMAPKGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1 (By similarity). 
Sequence Annotation
 DOMAIN 45 107 SH3.
 MOD_RES 362 362 Phosphoserine.
 MOD_RES 371 371 Phosphothreonine.
 MOD_RES 820 820 Phosphoserine.
 MOD_RES 1754 1754 Phosphoserine.
 MOD_RES 1764 1764 Phosphoserine.
 MOD_RES 1765 1765 Phosphoserine.
 MOD_RES 1770 1770 Phosphoserine (By similarity).
 MOD_RES 1915 1915 Phosphoserine.
 MOD_RES 1918 1918 Phosphoserine.
 CARBOHYD 360 360 N-linked (GlcNAc...) (Potential).
 CARBOHYD 631 631 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Endoplasmic reticulum; ER-Golgi transport; Exocytosis; Glycoprotein; Membrane; Phosphoprotein; Protein transport; Reference proteome; SH3 domain; Signal; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1930 AA 
Protein Sequence
MAAAPGLLFW LFVLGALWWV PGQSDLSHGR RFSDLKVCGD EECSMLMYRG KALEDFTGPD 60
CRFVNFKKGD DVYVYYKLAG GSLELWAGSV EHSFGYFPKD LIKVLHKYTE EELHIPADET 120
DFVCFEGGRD DFNSYNVEEL LGSLELEDSV PEESKKAEEV SQHREKSPEE SRGRELDPVP 180
EPEAFRADSE DGEGAFSEST EGLQGQPSAQ ESHPHTSGPA ANAQGVQSSL DTFEEILHDK 240
LKVPGSESRT GNSSPASVER EKTDAYKVLK TEMSLDLKTK FGSTADALVS DDEATRLVTS 300
LEDGFDEALD AEYYPMEEEE EVEEDADSSD ELPLLTFSDK DEKVPGKPMI EKYLTDKDPN 360
LSEEDKVEPP TWGDAFFSIV TGGEGKPGVV DLERSIEEEE DVSVSSSHQR KPQPAAGYTD 420
SEDEGDDLFV EEPKTNDVKD SETDPELVIT GEEKDIQESR KGLVQPESQS EDAKSETASA 480
YRLQGSKLNP LSAAEKGRDF TLKAVFEKKE NGLKESVIHI SKETLHEDKT REIQRDSLES 540
ELVHRALGSS VTENNKPKSL GVAPLLGNNK PDASKDSTEV PDGSVSGPKA GQQEGFLEPG 600
LKTQHQPRFS PPEETGPSRE LGGKVPISGR NLSWQQEQDV AAVVGKHANE KTGFPEEESR 660
EDGTDAEQAR AIRRPQEAES PEVLSVQPGR PDEEEEEEEG DNYPPEGLME DENAVSAQQS 720
RENSPSARDG RSDMNSQVFE KVILGTLNLN TEKTKQPANM ILETGQESET TSEEAGDVGK 780
ESGHSVVVDS EESHLADMRA QRPSQVHGLR DETAAQTPGS GEAVLSKNPN DLQKDNPEEE 840
LVNTLGLEDP GVGEISEGEP EDTKEFGVSE SQGTDAEDLR DDPSRQATPE IPDIVLKSIR 900
EDLPIINSFF KDDQQSLHRF LKYFDVRELE GLLEDMSIRL RSAHQNSLPY NMEKVLDKVF 960
RASESRILSM AEKMLDTGVA KNRDLGSKES SPLEEAEVLD DIQDLIYFVR YQYSGVETAP 1020
LVTPPPPEEG WARPGEERQP PQQDSLPQEN TGDLSVQPPE EPELSDQPVT SVQPPEEPEL 1080
SDQPVTSVQP PEEPELSDQP VTSVQPPEEP ELSDQPVTGY TSTSEVSQKP DTKKDIDLGP 1140
VMEGGPVGAG DVQKQLETIA EEPAAVPPLE SAFGSLYAFI LYLSKMLLAT LPDNVQPGPD 1200
FYGLPWQPVI ITAVLGIVSF AIFSWRTILV VKSRVYQVTE KQISEKLENI KKENAELMQK 1260
LSSYEQKIKE SKKYVQETKK QNMILSDEAV KYKDKIKILE ETNVSLGDKA KSLRLQLESE 1320
REQNVKNQDL ILENKKSIEK LKDVISMNAS ELSEVQVALN EAKLSEENVK SECHRVQEEN 1380
ARLKKKKEQL QQQVEEWSKS HAELTGQIKS FEKSQEDLEI ALTHKDDNIS ALTNCITQLN 1440
RLECELESED PDKGGNESDD LANGETGGDR SEKIRNRIKQ MMDVSRTQTA VSIVEEDLKL 1500
LQLKLRASMS TKCNLEDQIK KLEDDRSSLQ TAKAGLEDEC KTLRQKVEIL NELYQQKEMA 1560
LQKKLSQEEY ERQDREQRLT AADEKVVLAA EEVKTYKRRI EEMEEELQKT ERSFKNQIAA 1620
HEKKAHDNWL KARAAERAMA EEKREAANLR HKLLEMTQKM AMRQDEPVIV KPMPGRPNTQ 1680
NPPRRGLLSQ NGSFGPSPVS GGECSPPLPA EPPGRPLSAT LSRRDTPRSE FGSLDRHLPR 1740
PRWPSEASGK HSASDPGPAP VVNSSSRSSS PAKAVDEGKV NMAPKGPPPF PGVPLMGGPV 1800
PPPIRYGPPP QLCGGPFGPR PLPPPFVPGM HPPLGVREYA PGVLPGKRDL PLDPREFLPG 1860
HTPFRPPGSL GPREFFIPGT RLPPPTHGPQ EYPPPPPAVR DSLPSGPREE AKPASPSSVQ 1920
DRSQASKPTP 1930 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
 GO:0016021; C:integral to membrane; ISS:UniProtKB.
 GO:0002063; P:chondrocyte development; IMP:MGI.
 GO:0030199; P:collagen fibril organization; IMP:MGI.
 GO:0006887; P:exocytosis; ISS:UniProtKB.
 GO:0030501; P:positive regulation of bone mineralization; IMP:MGI.
 GO:0015031; P:protein transport; ISS:UniProtKB. 
Interpro
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF07653; SH3_2 
SMART
 SM00326; SH3 
PROSITE
 PS50002; SH3 
PRINTS