CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004450
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytoplasmic protein NCK1 
Protein Synonyms/Alias
 NCK adaptor protein 1; Nck-1; SH2/SH3 adaptor protein NCK-alpha 
Gene Name
 NCK1 
Gene Synonyms/Alias
 NCK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
286GNPWYYGKVTRHQAEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. 
Sequence Annotation
 DOMAIN 2 61 SH3 1.
 DOMAIN 115 165 SH3 2.
 DOMAIN 190 252 SH3 3.
 DOMAIN 282 376 SH2.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 91 91 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 105 105 Phosphotyrosine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH2 domain; SH3 domain; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 377 AA 
Protein Sequence
MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN 60
SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE 120
REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA 180
AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG 240
LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG 300
HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP 360
IFTSEQGEKL YLVKHLS 377 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0012506; C:vesicle membrane; IEA:Compara.
 GO:0008093; F:cytoskeletal adaptor activity; NAS:UniProtKB.
 GO:0004860; F:protein kinase inhibitor activity; IDA:UniProtKB.
 GO:0030159; F:receptor signaling complex scaffold activity; NAS:UniProtKB.
 GO:0007015; P:actin filament organization; IEA:Compara.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0016477; P:cell migration; IEA:Compara.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0060548; P:negative regulation of cell death; IDA:UniProtKB.
 GO:0030838; P:positive regulation of actin filament polymerization; IMP:UniProtKB.
 GO:0042102; P:positive regulation of T cell proliferation; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0007172; P:signal complex assembly; NAS:UniProtKB.
 GO:0042110; P:T cell activation; IMP:UniProtKB.
 GO:0050852; P:T cell receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR017304; Cytoplasmic_NCK.
 IPR000980; SH2.
 IPR001452; SH3_domain. 
Pfam
 PF00017; SH2
 PF00018; SH3_1 
SMART
 SM00252; SH2
 SM00326; SH3 
PROSITE
 PS50001; SH2
 PS50002; SH3 
PRINTS
 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.