CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012001
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromatin assembly factor 1 subunit B 
Protein Synonyms/Alias
 CAF-1 subunit B; Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7 
Gene Name
 CHAF1B 
Gene Synonyms/Alias
 CAF1A; CAF1P60; MPHOSPH7; MPP7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13CEIAWHNKEPVYSLDubiquitination[1]
45DTNVRIWKVEKGPDGubiquitination[1]
48VRIWKVEKGPDGKAIubiquitination[1]
53VEKGPDGKAIVEFLSubiquitination[1, 2, 3, 4]
67SNLARHTKAVNVVRFubiquitination[1, 2, 5]
99LWKVNDNKEPEQIAFubiquitination[1, 3, 5]
116EDEAQLNKENWTVVKubiquitination[1, 2, 5]
123KENWTVVKTLRGHLEubiquitination[5]
163WDVSKGQKISIFNEHubiquitination[1]
202LRVYSIQKKRVAFNVubiquitination[5]
203RVYSIQKKRVAFNVSubiquitination[1]
211RVAFNVSKMLSGIGAubiquitination[1, 4, 5, 6, 7]
234MFHDDSMKSFFRRLSubiquitination[1, 6, 7]
385DELGIPLKEKPVLNMubiquitination[6, 7]
387LGIPLKEKPVLNMRTubiquitination[3]
399MRTPDTAKKTKSQTHubiquitination[3]
494NTLQAWSKTTPRRINacetylation[8]
494NTLQAWSKTTPRRINubiquitination[1, 6, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. 
Sequence Annotation
 REPEAT 11 54 WD 1.
 REPEAT 64 103 WD 2.
 REPEAT 127 166 WD 3.
 REPEAT 169 208 WD 4.
 REPEAT 228 279 WD 5.
 REPEAT 299 340 WD 6.
 REPEAT 344 385 WD 7.
 MOD_RES 409 409 Phosphoserine.
 MOD_RES 419 419 Phosphothreonine.
 MOD_RES 429 429 Phosphoserine.
 MOD_RES 433 433 Phosphothreonine.
 MOD_RES 458 458 Phosphoserine.
 MOD_RES 494 494 N6-acetyllysine.
 MOD_RES 495 495 Phosphothreonine (Potential).
 MOD_RES 509 509 Phosphothreonine (Potential).
 MOD_RES 521 521 Phosphothreonine (Potential).
 MOD_RES 531 531 Phosphothreonine (Potential).
 MOD_RES 538 538 Phosphoserine.  
Keyword
 Acetylation; Cell cycle; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; DNA replication; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 559 AA 
Protein Sequence
MKVITCEIAW HNKEPVYSLD FQHGTAGRIH RLASAGVDTN VRIWKVEKGP DGKAIVEFLS 60
NLARHTKAVN VVRFSPTGEI LASGGDDAVI LLWKVNDNKE PEQIAFQDED EAQLNKENWT 120
VVKTLRGHLE DVYDICWATD GNLMASASVD NTAIIWDVSK GQKISIFNEH KSYVQGVTWD 180
PLGQYVATLS CDRVLRVYSI QKKRVAFNVS KMLSGIGAEG EARSYRMFHD DSMKSFFRRL 240
SFTPDGSLLL TPAGCVESGE NVMNTTYVFS RKNLKRPIAH LPCPGKATLA VRCCPVYFEL 300
RPVVETGVEL MSLPYRLVFA VASEDSVLLY DTQQSFPFGY VSNIHYHTLS DISWSSDGAF 360
LAISSTDGYC SFVTFEKDEL GIPLKEKPVL NMRTPDTAKK TKSQTHRGSS PGPRPVEGTP 420
ASRTQDPSSP GTTPPQARQA PAPTVIRDPP SITPAVKSPL PGPSEEKTLQ PSSQNTKAHP 480
SRRVTLNTLQ AWSKTTPRRI NLTPLKTDTP PSSVPTSVIS TPSTEEIQSE TPGDAQGSPP 540
ELKRPRLDEN KGGTESLDP 559 
Gene Ontology
 GO:0033186; C:CAF-1 complex; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; NAS:UniProtKB.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0003682; F:chromatin binding; TAS:ProtInc.
 GO:0042393; F:histone binding; NAS:UniProtKB.
 GO:0051082; F:unfolded protein binding; TAS:ProtInc.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0006335; P:DNA replication-dependent nucleosome assembly; TAS:ProtInc.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001632; Gprotein_B.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00319; GPROTEINB.